Mouse Angiopoietin-related protein 6(ANGPTL6) ELISA kit

Mouse Angiopoietin-related protein 6(ANGPTL6) ELISA kit

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中文名称：

小鼠血管生成素相关蛋白6(ANGPTL6)酶联免疫试剂盒
货号：

CSB-EL001714MO
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO小鼠血管生成素相关蛋白6（ANGPTL6）酶联免疫吸附检测试剂盒（货号：CSB-EL001714MO），基于双抗体夹心法原理设计，适用于血清、血浆及组织匀浆样本中ANGPTL6蛋白的定量检测，检测灵敏度范围为62.5 pg/mL至4000 pg/mL。ANGPTL6是血管生成素样蛋白家族成员，在糖脂代谢调控、血管生成及组织修复等生理过程中发挥重要作用，其表达水平与肥胖、糖尿病及心血管疾病等研究密切相关。试剂盒包含预包被特异性抗体的96孔板、生物素标记检测抗体、标准品及显色底物等核心组分，操作时仅需4小时即可完成样本孵育、洗涤和显色步骤，标准品经梯度稀释后可生成高线性标准曲线（R²≥0.99），确保检测结果准确可靠。该试剂盒支持-20°C保存12个月，适用于基础科研中探索ANGPTL6在代谢性疾病模型、血管生成机制或相关信号通路中的表达变化，为体外实验提供高效定量分析工具。
别名：

Angptl6 ELISA kit; AgfAngiopoietin-related protein 6 ELISA kit; Angiopoietin-like protein 6 ELISA kit; Angiopoietin-related growth factor ELISA kit
缩写：

ANGPTL6
Uniprot No.：

Q8R0Z6
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

62.5 pg/mL-4000 pg/mL
灵敏度：

15.6 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ANGPTL6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	89
	Range %	82-96
1:200	Average %	98
	Range %	93-102
1:400	Average %	99
	Range %	95-107
1:800	Average %	91
	Range %	87-95

回收率：

The recovery of mouse ANGPTL6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	84-95
EDTA plasma (n=4)	99	96-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.616	2.793	2.705	2.546
2000	1.927	2.036	1.982	1.823
1000	1.287	1.388	1.338	1.179
500	0.769	0.729	0.749	0.590
250	0.577	0.604	0.591	0.432
125	0.394	0.401	0.398	0.239
62.5	0.247	0.267	0.257	0.098
0	0.164	0.154	0.159

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Leptin regulates the expression of angiopoietin-like 6 Min-Jeong Kim.et al,Biochemical and Biophysical Research Communications,2018
ANGPTL6 expression is coupled with mitochondrial OXPHOS function to regulate adipose FGF21 Seul Gi Kang.et al,Endocrinology.,2017

功能：

May play a role in the wound healing process. May promote epidermal proliferation, remodeling and regeneration. May promote the chemotactic activity of endothelial cells and induce neovascularization. May counteract high-fat diet-induced obesity and related insulin resistance through increased energy expenditure.
基因功能参考文献：
1. Since upregulation of ANGPTL6 is induced on metabolic stress, we investigated the hepatic expression of ANGPTL6 by leptin, a representative adipokine of obesity. Mice on a high-fat diet showed increased serum leptin levels and hepatic Angptl6 expression. PMID: 29852166
2. Upregulation of ANGPTL6 in mouse keratinocytes enhances susceptibility to psoriasis. PMID: 27698489
3. mitochondrial OXPHOS function governs the expression of ANGPTL6, which is an essential factor for FGF21 production in adipose tissue and cultured adipocytes. PMID: 28184000
4. In addition to pronounced angiogenesis and epidermal hyperplasia, marked enlargement of dermal lymphatic vessels was observed in UV-B-exposed Angptl6/AGF transgenic mice. PMID: 22132739
5. we show that (>80%) of the AGF-deficient mice die at about embryonic day 13, while the surviving AGF-deficient mice develop marked obesity, lipid accumulation in skeletal muscle and liver, and insulin resistance accompanied by reduced energy expenditure PMID: 15778720
6. Our results strongly suggest that AGF is an integrin ligand as well as a mitogenic growth factor and theoretically participates in cutaneous wound healing in a more complex mechanism. PMID: 16806062
7. Activation of an ERK1/2-eNOS-NO pathway is a crucial signaling mechanism by which angiopoietin-related growth factor increases blood flow through induction of angiogenesis and arteriogenesis. PMID: 18258819
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亚细胞定位：

Secreted.
组织特异性：

Highly expressed in the liver, specifically in hepatocytes, and weakly in the heart. Expressed in hematopoietic cells, platelets and mast cells, and detected at wounded skin.
数据库链接：

KEGG: mmu:70726

STRING: 10090.ENSMUSP00000035784

UniGene: Mm.34019

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.