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  4. Mouse Alpha-fetoprotein,AFP ELISA Kit

Mouse Alpha-fetoprotein,AFP ELISA Kit

文献引用 (5) 说明书下载
  • 中文名称：
    小鼠甲种胎儿球蛋白/甲胎蛋白(AFP)酶联免疫试剂盒
  • 货号：
    CSB-E08282m
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO小鼠甲种胎儿球蛋白/甲胎蛋白（AFP）酶联免疫检测试剂盒（货号：CSB-E08282m），基于双抗体夹心法原理实现定量检测。AFP作为胚胎发育阶段由肝脏和卵黄囊分泌的重要糖蛋白，在成年个体中通常维持低水平表达，但在肝细胞癌、肝再生及特定病理模型中呈现显著升高特征，是研究肝脏疾病机制及肿瘤模型的常用生物标志物。本试剂盒可精准检测小鼠血清、血浆及组织匀浆样本中的AFP浓度，灵敏度下限为1.23 ng/ml，检测线性范围覆盖1.23-900 ng/ml，适用于体外定量分析。其特异性抗体组合确保对目标蛋白的高效捕获与信号放大，配套试剂经严格验证，操作流程稳定可靠。科研人员可通过该试剂盒探究肝损伤修复、肿瘤发生发展或胚胎发育相关机制，为构建疾病模型、评估干预效果提供数据支持。本产品仅限科学研究使用，不适用于临床诊断及其他非科研用途。
  • 别名：
    AfpAlpha-fetoprotein ELISA kit; Alpha-1-fetoprotein ELISA kit; Alpha-fetoglobulin ELISA kit
  • 缩写：
    AFP
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates.
  • 检测范围：
    1.23 ng/ml-900 ng/ml.
  • 灵敏度：
    1.23 ng/ml.
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

    　

    Intra-Assay Precision

    Inter-Assay Precision

    Sample

    1

    2

    3

    1

    2

    3

    n

    20

    20

    20

    20

    20

    20

    Mean(ng/ml)

    34.056

    34.846

    35.720

    33.819

    34.253

    34.648

    SD

    0.035

    0.038

    0.042

    0.036

    0.042

    0.053

    CV(%)

    4.481

    4.744

    5.103

    4.645

    5.344

    6.658

  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse AFP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    86

    Range %

    82-93

    1:2

    Average %

    98

    Range %

    94-104

    1:4

    Average %

    99

    Range %

    96-108

    1:8

    Average %

    88

    Range %

    80-100

  • 回收率：

    The recovery of mouse AFP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    91

    83-97

    EDTA plasma (n=4)

    96

    92-105

  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    900

    2.379

    2.252

    2.316

    2.161

    300

    1.975

    1.876

    1.926

    1.771

    100

    1.305

    1.304

    1.305

    1.150

    33.33

    0.812

    0.779

    0.796

    0.641

    11.11

    0.534

    0.494

    0.514

    0.359

    3.7

    0.372

    0.349

    0.361

    0.206

    1.23

    0.264

    0.249

    0.257

    0.102

    0

    0.161

    0.149

    0.155

    　

  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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Afp antibodies

Afp Proteins

靶点详情

  • 功能：
    Binds estrogens, fatty acids and metals.
  • 基因功能参考文献：
    1. endogenous AFP expression in the perinatal liver is reduced in the absence of beta-catenin, suggesting that beta-catenin regulates AFP during liver development. PMID: 22544812
    2. Findings indicate that AFP expressed under experimental cryptorchid conditions plays a role as a regulatory factor in spermatogenesis and in hepatic generation. PMID: 21573244
    3. AFP mRNA and VEGF-C mRNA in peripheral blood were significantly correlated with recurrence and metastasis of hepatocellular carcinoma. PMID: 20506647
    4. A DEHP treatment-induced phenotype was identified with lack of glycogen accumula-tion and intracytoplasmic localization of beta-catenin which was associated with increased AFP gene expression. PMID: 20307648
    5. The deficits observed in parental behavior of AFP knockout females could not be explained by any changes in olfactory function, novelty recognition or anxiety PMID: 20109458
    6. Foxa1 acts as a pioneer transcription factor in de novo activation of Afp, by exploiting a lack of methylation at juxtaposed transposed elements PMID: 20348100
    7. The level of AFP mRNA in peripheral blood may indicate recurrence and/or metastasis after curative resection of hepatocellular carcinoma. PMID: 12113675
    8. AFP affects cell proliferation by binding its receptor to trigger the signal transduction pathway of cAMP-PKA and alter the expression of K- ras p21 genes PMID: 12118941
    9. A murine Nkx2.8 was isolated from the Hepal-6 cell line and showed oligonucleotide binding competitive with fetoprotein transcription factor. PMID: 12167706
    10. The mouse alpha-fetoprotein promoter is repressed in HepG2 hepatoma cells by hepatocyte nuclear factor-3 PMID: 12215259
    11. AFP has a role in female fertility, but not in male fertility or survival, in mice PMID: 12297623
    12. mouse and human alpha-fetoprotein enhancers are strikingly different PMID: 15028291
    13. AFP protects the developing female brain from the adverse effects of prenatal estrogen exposure and clarify a long-running debate on the role of this fetal protein in brain sexual differentiation PMID: 16479017
    14. 30-kDa protein is a novel isoform of AUF1 family and is the main component of the DAP-II complex that binds to the DAS sequence PMID: 16514630
    15. The sex difference in the ability to show preovulatory LH surges depends on the prenatal actions of estrogens in the male hypothalamus, is lost in Afp(-/-) females. PMID: 18202134
    16. data indicate that this enhancer region is required for alpha-fetoprotein and albumin activation early in liver development and alpha-fetoprotein reactivation during liver regeneration PMID: 19782060

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    ALB/AFP/VDB family
  • 组织特异性：
    Plasma.
  • 数据库链接：

    KEGG: mmu:11576

    STRING: 10090.ENSMUSP00000041006

    UniGene: Mm.358570

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