Your Good Partner in Biology Research

中文简体

CSF2｜ CTLA4｜ DLL3｜ EGFR｜ IL4｜ IL4R｜ LAG3｜ MSTN｜ NECTIN4｜ NT5E｜ SEZ6｜ TM4SF1｜ TNFSF13｜ TPO

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Mouse Alpha-enolase(ENO1) ELISA kit

Mouse Alpha-enolase(ENO1) ELISA kit

说明书下载
  • 中文名称：
    小鼠烯醇酶1(ENO1)酶联免疫试剂盒
  • 货号：
    CSB-EL007670MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO小鼠烯醇酶1（ENO1）酶联免疫检测试剂盒（货号CSB-EL007670MO），基于双抗体夹心法原理开发，可定量检测血清、血浆及组织匀浆样本中的ENO1蛋白水平，覆盖62.5 pg/mL至4000 pg/mL的宽检测范围。ENO1作为糖酵解途径的关键酶，广泛参与细胞能量代谢调控，其表达异常与肿瘤微环境重塑、免疫应答调控等病理生理过程密切相关，是代谢性疾病及癌症机制研究的重要靶标。试剂盒采用高亲和力抗体对实现特异性捕获与检测，实验流程标准化，适用于体外定量分析小鼠模型或细胞实验中ENO1的动态表达变化，可为代谢通路研究、肿瘤生长微环境分析、炎症反应机制探索等基础科研领域提供可靠数据支持。样本兼容性方面，试剂盒支持直接检测液态样本（血清、血浆），组织样本需经匀浆及离心预处理后取上清检测。所有组分均经严格验证，确保批间一致性与检测稳定性。
  • 别名：
    Eno1 ELISA Kit; Eno-1 ELISA Kit; Alpha-enolase ELISA Kit; EC 4.2.1.11 ELISA Kit; 2-phospho-D-glycerate hydro-lyase ELISA Kit; Enolase 1 ELISA Kit; Non-neural enolase ELISA Kit; NNE ELISA Kit
  • 缩写：
    ENO1
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    62.5 pg/mL-4000 pg/mL
  • 灵敏度：
    15.6 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse ENO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 98
    Range % 94-103
    1:2 Average % 85
    Range % 81-90
    1:4 Average % 104
    Range % 100-107
    1:8 Average % 85
    Range % 80-90
  • 回收率：
    The recovery of mouse ENO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 107 103-110
    EDTA plasma (n=4) 86 83-89
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    4000 2.557 2.601 2.579 2.416
    2000 2.291 2.186 2.239 2.076
    1000 1.553 1.562 1.558 1.395
    500 0.981 0.998 0.990 0.827
    250 0.582 0.571 0.577 0.414
    125 0.414 0.432 0.423 0.260
    62.5 0.295 0.290 0.293 0.130
    0 0.161 0.164 0.163 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

产品评价

相关产品

Eno1 antibodies

Eno1 Proteins

靶点详情

  • 功能：
    Glycolytic enzyme the catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. In addition to glycolysis, involved in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
  • 基因功能参考文献：
    1. this paper shows the immunoregulatory role of alpha enolase in dendritic cell function during Chlamydia infection PMID: 28525970
    2. Eno1 may be a potential marker for low sperm motility induced by iron ion radiation, and these results may provide a useful reference for changes in astronaut fertility during long space missions. PMID: 27460355
    3. The results confirmed the altered expression of HFABP, a key fatty acid transport protein, and of enolase and PGK1, the key enzymes in the glycolytic process PMID: 27966485
    4. Pre-immunization with ENO1 or its immunodominant peptide pEP1 reduces CIA severity at the clinical, immunological and histological levels PMID: 26302382
    5. a Wnt/beta-catenin-driven functional role of ENO1 and PDIA3 in alveolar epithelial cell plasticity in lung injury and repair, is reported. PMID: 26035385
    6. Enolase is a new target of liver X receptors. PMID: 25708389
    7. Alpha-enolase is upregulated on the cell surface and responds to plasminogen activation in mice expressing a 133p53alpha mimic PMID: 25643152
    8. MBP1 inhibits Ab fibril formation in vitro and demonstrate the ability of MBP1 to reduce Ab pathology and improve behavioral performance. PMID: 25457550
    9. These data show a multiantibody composition in lupus nephritis, where IgG2 autoantibodies against alpha-enolase and annexin AI predominate in the glomerulus and can be detected in serum. PMID: 24790181
    10. These results demonstrate the novel requirement of alpha-enolase for restoring homeostasis of injured muscle tissue, by controlling the pericellular localization of plasmin activity. PMID: 23239981
    11. ENO1-positive monocytes and macrophages are found in arthritic ankle tissues of mice with collagen-induced arthritis. PMID: 22623332
    12. By concentrating and enhancing plasmin generation on the cell surface, alpha-enolase-type plasminogen receptor is an important component of skeletal myogenesis. PMID: 14515195
    13. the expression of alpha-enolase plasminogen receptor may serve to concentrate and enhance plasmin generation on the cell surface of migratory myoblasts contributing to efficient muscle repair [review] PMID: 15574344
    14. plasminogen stimulated DNA binding activity of the transcription factors activator-protein 1 and early growth response gene-1 to their cognate regulatory sequences at alpha-enolase promoter PMID: 16225843

    显示更多

    收起更多

  • 亚细胞定位：
    Cytoplasm. Cell membrane.
  • 蛋白家族：
    Enolase family
  • 组织特异性：
    Testis. Found in the principal piece of sperm tail (at protein level). The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma het
  • 数据库链接：

    KEGG: mmu:13806

    STRING: 10090.ENSMUSP00000079727

    UniGene: Mm.372357

Most popular with customers

产品中心
特别关注


X
九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |
九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |