Mouse Alanine Aminotransferase(ALT/GPT)ELISA Kit

Mouse Alanine Aminotransferase(ALT/GPT)ELISA Kit

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中文名称：

小鼠丙氨酸转氨酶/谷丙转氨酶(ALT/GPT)酶联免疫试剂盒
货号：

CSB-E16539m
规格：

96T/48T
价格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO小鼠丙氨酸转氨酶/谷丙转氨酶（ALT/GPT）酶联免疫检测试剂盒（货号：CSB-E16539m），采用双抗体夹心法原理实现定量分析，可精准检测血清、血浆、细胞培养上清及细胞裂解液样本中ALT/GPT的活性水平。ALT作为肝脏功能的关键酶，在肝细胞损伤时大量释放至血液，是评估肝脏健康与疾病研究的常用生物标志物。本试剂盒检测灵敏度达0.156 mU/mL，线性范围为0.156-10 mU/mL，适用于低浓度样本的精确检测。实验操作采用预包被特异性抗体的96孔板，通过标准品梯度稀释建立标准曲线，结合显色反应实现目标酶活性定量。该试剂盒广泛应用于肝损伤模型评估、药物肝毒性研究、代谢性疾病机制探索等科研领域，特别适用于体外培养肝细胞或动物模型中肝脏相关病理生理变化的动态监测，为肝脏功能研究提供可靠的实验工具。检测流程包含样本预处理、孵育、洗涤及数据读取步骤，提供完整的实验方案与配套试剂，确保实验便捷性和结果可重复性。
别名：

Gpt ELISA kit; Gpt1Alanine aminotransferase 1 ELISA kit; ALT1 ELISA kit; EC 2.6.1.2 ELISA kit; Glutamate pyruvate transaminase 1 ELISA kit; GPT 1 ELISA kit; Glutamic--alanine transaminase 1 ELISA kit; Glutamic--pyruvic transaminase 1 ELISA kit
缩写：

ALT/GPT
Uniprot No.：

Q8QZR5
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell culture supernates, cell lysates
检测范围：

0.156 mU/mL-10 mU/mL
灵敏度：

0.039 mU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ALT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	83-95
1:2	Average %	97
	Range %	92-101
1:4	Average %	106
	Range %	98-113
1:8	Average %	94
	Range %	87-97

回收率：

The recovery of mouse ALT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-97
EDTA plasma (n=4)	102	95-108

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mU/ml	OD1	OD2	Average	Corrected
10	2.645	2.566	2.606	2.421
5	2.015	1.855	1.935	1.750
2.5	1.194	1.155	1.175	0.990
1.25	0.777	0.796	0.787	0.602
0.625	0.492	0.461	0.477	0.292
0.312	0.335	0.344	0.340	0.155
0.156	0.278	0.288	0.283	0.098
0	0.191	0.178	0.185

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

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功能：

Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles.
基因功能参考文献：
1. Dexamethasone induces a metabolic adaptive ALT isoenzyme response in livers of C57/BL6 mice. PMID: 22609950
2. PKCdelta activation and protein levels are elevated in an animal model of steatohepatitis, which was recapitulated in a cell model, supporting the conclusion that PKCdelta plays a role in ALT release, the ER stress signal, and cell death PMID: 20971848
3. In experiments where mice were provided ethanol chronically in drinking water, where stress is minimal, higher levels of ALT were noted in animals in the ethanol group as compared with animals in the control group. PMID: 15652761
亚细胞定位：

Cytoplasm.
蛋白家族：

Class-I pyridoxal-phosphate-dependent aminotransferase family, Alanine aminotransferase subfamily
组织特异性：

Mainly expressed in liver, intestine, colon and white adipose tissue.
数据库链接：

KEGG: mmu:76282

STRING: 10090.ENSMUSP00000023203

UniGene: Mm.30130

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.