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中文名称:小鼠活化素B(ACV-B)酶联免疫试剂盒
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货号:CSB-E15932m
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规格:96T/48T
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价格:¥3600/¥2500
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其他:
产品详情
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产品描述:小鼠活化素B(ACV-B)酶联免疫试剂盒(CSB-E15932m)为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的ACV-B含量。试剂盒检测范围为6.25 pg/mL-400 pg/mL,适用于实验室研究,可广泛用于评估动物模型中ACV-B在卵巢功能、胚胎形成、代谢综合征(如多囊卵巢或肥胖模型)中的表达变化,亦可用于探索其在组织再生或炎症反应中的调控机制。实验兼容多种生物样本类型,为体外研究提供灵活选择本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
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缩写:ACV-B
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种属:Mus musculus (Mouse)
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样本类型:serum, plasma, tissue homogenates
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检测范围:6.25 pg/mL-400 pg/mL
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灵敏度:1.56 pg/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Others
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测定原理:quantitative
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测定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ACV-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 98 Range % 92-105 1:2 Average % 94 Range % 85-100 1:4 Average % 91 Range % 86-98 1:8 Average % 96 Range % 91-104 -
回收率:
The recovery of mouse ACV-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 92 90-95 EDTA plasma (n=4) 95 92-98 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 400 2.278 2.252 2.265 2.179 200 2.132 2.015 2.074 1.988 100 1.612 1.641 1.627 1.541 50 0.987 0.933 0.960 0.874 25 0.541 0.522 0.532 0.446 12.5 0.278 0.285 0.282 0.196 6.25 0.184 0.194 0.189 0.103 0 0.085 0.087 0.086 -
数据处理:
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货期:3-5 working days
引用文献
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