Mouse β-hexosaminidase A,β-Hex A ELISA Kit
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中文名称:小鼠β氨基己糖苷酶A(β-Hex A)酶联免疫试剂盒
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货号:CSB-E04818m
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规格:96T/48T
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价格:¥3800/¥2500
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其他:
产品详情
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产品描述:小鼠β氨基己糖苷酶A(β-Hex A)酶联免疫试剂盒(CSB-E04818m)为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的HEXA含量。HEXA是一种溶酶体酶,负责分解GM2神经节苷脂,维持细胞内环境稳定。其突变会导致GM2神经节苷脂在细胞内积累,引发泰萨二氏病等疾病。研究HEXA的机制,旨在开发治疗相关遗传性神经退行性疾病的药物。试剂盒检测范围为3.12 μU/mL-200 μU/mL,适用于研究β-Hex A在代谢性疾病模型小鼠中的表达调控、基因敲除/过表达动物模型的酶活性验证,以及药物干预或病理条件下溶酶体功能的评估等科研场景。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
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别名:Hexa ELISA Kit; Beta-hexosaminidase subunit alpha ELISA Kit; EC 3.2.1.52 ELISA Kit; Beta-N-acetylhexosaminidase subunit alpha ELISA Kit; Hexosaminidase subunit A ELISA Kit; N-acetyl-beta-glucosaminidase subunit alpha ELISA Kit
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缩写:
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Uniprot No.:
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种属:Mus musculus (Mouse)
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样本类型:serum, plasma, tissue homogenates
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检测范围:3.12 μU/mL-200 μU/mL
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灵敏度:0.78 μU/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Neuroscience
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测定原理:quantitative
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测定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse β-Hex A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:10 Average % 92 Range % 85-100 1:20 Average % 93 Range % 87-98 1:40 Average % 90 Range % 86-100 1:80 Average % 90 Range % 86-98 -
回收率:
The recovery of mouse β-Hex A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 97 89-101 EDTA plasma (n=4) 95 92-98 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. μU/ml OD1 OD2 Average Corrected 200 2.542 2.517 2.530 2.386 100 2.073 2.101 2.087 1.943 50 1.452 1.464 1.458 1.314 25 0.942 0.961 0.952 0.808 12.5 0.416 0.402 0.409 0.265 6.25 0.325 0.311 0.318 0.174 3.12 0.247 0.237 0.242 0.098 0 0.146 0.141 0.144 -
数据处理:
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货期:3-5 working days
相关产品
靶点详情
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功能:Hydrolyzes the non-reducing end N-acetyl-D-hexosamine and/or sulfated N-acetyl-D-hexosamine of glycoconjugates, such as the oligosaccharide moieties from proteins and neutral glycolipids, or from certain mucopolysaccharides. The isozyme S is as active as the isozyme A on the anionic bis-sulfated glycans, the chondroitin-6-sulfate trisaccharide (C6S-3), and the dermatan sulfate pentasaccharide, and the sulfated glycosphingolipid SM2. The isozyme B does not hydrolyze each of these substrates, however hydrolyzes efficiently neutral oligosaccharide. Only the isozyme A is responsible for the degradation of GM2 gangliosides in the presence of GM2A.
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基因功能参考文献:
- Neu4 is not the only sialidase contributing to the metabolic bypass in Hexa(-/-) mice. PMID: 20862357
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亚细胞定位:Lysosome.
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蛋白家族:Glycosyl hydrolase 20 family
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组织特异性:Ubiquitous. Most abundant in testis, adrenal, epididymis and heart. Low levels seen in the liver.
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数据库链接:
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