Monkey Tumor necrosis factor α,TNF-α ELISA KIT

Monkey Tumor necrosis factor α,TNF-α ELISA KIT

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中文名称：

猴肿瘤坏死因子α(TNF-α)酶联免疫试剂盒
货号：

CSB-E10039Mo
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

本试剂盒（CSB-E10039Mo）采用双抗体夹心法对猴源肿瘤坏死因子α（TNF-α）进行定量检测，该靶标作为促炎细胞因子在免疫应答、炎症反应及细胞凋亡等生理病理过程中具有核心调控作用，常用于灵长类动物感染性疾病、自身免疫模型及炎症机制研究。试剂盒基于预包被高特异性抗体的固相载体，结合生物素标记检测抗体与HRP标记链霉亲和素系统，可精准检测血清、血浆或组织匀浆样本中1.56-100 pg/mL浓度范围内的TNF-α蛋白水平，最低检测限达1.56 pg/mL，满足低丰度靶标分析需求。该产品适用于恒河猴、食蟹猴等非人灵长类动物的基础研究，如病毒感染模型中细胞因子风暴评估、免疫抑制剂作用机制探索，或通过组织样本分析特定器官炎症微环境变化，为神经退行性疾病、代谢综合征等科研方向提供关键数据支持。试剂盒配套标准品及优化缓冲体系，操作流程可在4.5小时内完成，适用于实验室常规蛋白定量分析。
别名：

TNF; TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
缩写：

TNF
Uniprot No.：

P48094
种属：

Monkey
样本类型：

serum, plasma, tissue homogenates
检测范围：

1.56 pg/mL-100 pg/mL
灵敏度：

0.39 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
100	2.105	2.097	2.101	2.008
50	1.863	1.805	1.834	1.741
25	1.412	1.395	1.404	1.311
12.5	0.909	0.896	0.903	0.810
6.25	0.594	0.549	0.572	0.479
3.12	0.306	0.298	0.302	0.209
1.56	0.191	0.187	0.189	0.096
0	0.096	0.090	0.093

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
基因功能参考文献：
1. These interactions result in the induction of the TNF signaling pathway, activation of apoptosis, and DNA-damage stress response. PMID: 24743303
2. Findings showed that both mucosal compartments harbor similar percentages of memory CD4(+) T cells and displayed comparable cytokine TNF-alpha responses to mitogenic stimulations prior to infection. PMID: 24610016
3. Primary role for IL-1beta and TNF-alpha in the triggering of preterm labor associated with inflammation or infection. PMID: 17132473
亚细胞定位：

Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, membrane form]: Membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, soluble form]: Secreted.; [C-domain 1]: Secreted.; [C-domain 2]: Secreted.
蛋白家族：

Tumor necrosis factor family
数据库链接：

KEGG: mcc:715467

STRING: 9544.ENSMMUP00000011594

UniGene: Mmu.3364

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.