Monkey Interleukin 15,IL-15 ELISA Kit

产品详情

产品描述：

CUSABIO专用于灵长类动物研究的猴子白介素15（IL-15）酶联免疫检测试剂盒（货号CSB-E14392Mk），基于双抗体夹心法原理实现对血清、血浆样本中IL-15蛋白的定量检测。IL-15作为重要的免疫调节因子，在T细胞增殖、NK细胞活化及抗病毒免疫应答中发挥关键作用，其水平变化常被用于评估灵长类动物感染性疾病模型、疫苗免疫效果或免疫治疗研究中的免疫状态。CUSABIO试剂盒采用高特异性配对抗体，检测灵敏度达15.6 pg/mL，线性范围覆盖15.6-1000 pg/mL，可在标准酶标仪（450nm波长）下实现稳定检测。预包被96孔板经过优化处理，支持直接加载稀释后的体液样本，配合即用型标准品与显色试剂，可在3.5小时内完成实验流程。该产品适用于非人灵长类动物（如食蟹猴、恒河猴）的免疫机制研究、炎症性疾病模型构建及生物制剂药效评价等科研领域，为探索IL-15在先天性与适应性免疫中的调控作用提供可靠工具。
别名：

IL15 ELISA Kit; Interleukin-15 ELISA Kit; IL-15 ELISA Kit
缩写：

IL15
Uniprot No.：

P48092
种属：

Monkey
样本类型：

serum, plasma
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL15 requires interaction of IL15 with components of the IL2 receptor, including IL2RB and probably IL2RG but not IL2RA. In neutrophils, stimulates phagocytosis probably by signaling through the IL15 receptor, composed of the subunits IL15RA, IL2RB and IL2RG, which results in kinase SYK activation.
基因功能参考文献：
1. IL 15 generates a dramatic expansion of short-lived memory CD8 T cells and natural killer in immunocompetent macaques and has long-term effects on the balance of CD4(+) and CD8(+) T cells. PMID: 20631381
2. These data suggest that therapeutic use of IL-15 in the setting of antiretroviral therapy might facilitate specific restoration of the CD4 + T cell compartment. PMID: 16691294
3. IL-15 secretion significantly correlates with the up-regulated expression of CD4 on memory CD4 T cells that is associated with increased permissiveness to simian immunodeficiency virus infection. PMID: 19155491
亚细胞定位：

Secreted.
蛋白家族：

IL-15/IL-21 family
数据库链接：

KEGG: mcc:699616

STRING: 9544.ENSMMUP00000025100

UniGene: Mmu.3414