Monkey Interferon γ,IFN-γ ELISA Kit

Monkey Interferon γ,IFN-γ ELISA Kit

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中文名称：

猴γ干扰素(IFN-γ)酶联免疫试剂盒
货号：

CSB-E10049Mo
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO猴γ干扰素（IFN-γ）酶联免疫检测试剂盒（货号CSB-E10049Mo），采用双抗体夹心法原理实现定量检测，适用于科研领域对血清、血浆及组织匀浆样本中IFN-γ蛋白水平的精准分析。IFN-γ是由活化T细胞和NK细胞分泌的关键细胞因子，在先天性和适应性免疫应答中发挥核心调控作用，其浓度变化与炎症反应、病原体清除及免疫调节密切相关。试剂盒检测灵敏度覆盖31.25至2000 pg/mL的宽动态范围，可满足不同浓度样本的检测需求，标准曲线采用梯度浓度设计确保数据可靠性。实验操作兼容常规酶标仪，配套试剂包含预包被捕获抗体、生物素标记检测抗体及链霉亲和素-HRP系统，支持科研人员开展非人灵长类动物模型的免疫机制研究、感染性疾病模型评估或药物干预实验中的细胞因子动态监测。该试剂盒严格验证批次间稳定性，提供详尽的实验方案和标准化质控品，为体外实验提供高效稳定的检测工具。
别名：

IFNG ELISA Kit; Interferon gamma ELISA Kit; IFN-gamma ELISA Kit
缩写：

IFNG
Uniprot No.：

P63310
种属：

Monkey
样本类型：

serum, plasma, tissue homogenates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.8 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	1.830	1.990	1.910	1.805
1000	1.496	1.398	1.447	1.342
500	1.133	1.138	1.136	1.031
250	0.932	0.902	0.917	0.812
125	0.544	0.535	0.540	0.435
62.5	0.212	0.226	0.219	0.114
31.25	0.117	0.111	0.114	0.009
0	0.106	0.104	0.105

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL. Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation.
基因功能参考文献：
1. IFNgamma expression in colonic epithelial cells was regulated by TGFB1. PMID: 25769244
2. Higher KIR2DL4 copy numbers is associated with an increased IFN-gamma production in NK cell subsets in SIV-infected Mamu-A*01-negative rhesus macaques. PMID: 23449795
3. Data show that viral set-point in simian immunodeficiency virus diseasewas is associated with expression of interferon gamma -stimulated genes. PMID: 17719201
4. Low Sociable animals showed alterations in lymph node expression of the immunoregulatory cytokine interferon-gamma. PMID: 18068331
亚细胞定位：

Secreted.
蛋白家族：

Type II (or gamma) interferon family
组织特异性：

Released primarily from activated T lymphocytes.
数据库链接：

KEGG: mcc:574282

STRING: 9544.ENSMMUP00000025266

UniGene: Mmu.3373

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.