Human γ-Secretase ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CSB-E13495h人γ分泌酶酶联免疫试剂盒采用双抗体夹心法原理，专为血清、血浆及组织匀浆样本中γ分泌酶的精准定量分析而设计。γ分泌酶作为跨膜蛋白酶复合物，在阿尔茨海默病相关的β淀粉样蛋白生成通路中发挥关键作用，其活性与表达水平的检测对神经退行性疾病机制研究具有重要意义。本试剂盒检测灵敏度高，线性范围为31.25-2000 pg/mL，覆盖从基础研究到药物开发所需的广泛浓度梯度，预包被的高特异性抗体可确保对目标分子的高效捕获与检测。该产品适用于神经科学领域的基础研究，例如阿尔茨海默病动物模型的组织样本分析、体外培养细胞中γ分泌酶活性调控实验，或药理学研究中抑制剂/激活剂对酶表达水平影响的动态监测。试剂盒提供标准品及优化反应体系，支持用户在常规实验室条件下完成稳定、可重复的定量检测，为疾病机制探索及治疗策略开发提供可靠工具。
别名：

gamma-secretase
缩写：

γ-Secretase
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.8 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human γ-Secretase in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
Range %	86-97
1:2	Average %	97
Range %	91-103
1:4	Average %	95
Range %	89-100
1:8	Average %	94
Range %	89-98

回收率：

The recovery of human γ-Secretase spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	98-101
EDTA plasma (n=4)	98	95-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.