Human very low density lipoprotein(VLDL)ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

人极低密度脂蛋白(VLDL)酶联免疫试剂盒（CSB-E17087h）为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的VLDL含量。VLDL（极低密度脂蛋白）是一种运载内源性甘油三酯的脂蛋白。其水平异常与心血管疾病等密切相关。研究机制主要聚焦于VLDL在肝脏合成、代谢过程，以及其与受体相互作用，以明确其在脂质代谢紊乱和疾病发生发展中的具体作用。试剂盒检测范围为0.78 μg/mL-50 μg/mL，适用于科研领域中对脂代谢机制、心血管疾病动物模型的研究，也可用于药物干预或基因编辑后体内VLDL水平变化的动态监测，为代谢相关疾病的机制探索及生物标志物筛选提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

N/A
缩写：

VLDL
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.78 μg/mL-50 μg/mL
灵敏度：

0.195 μg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human VLDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	97
	Range %	91-103
1:400	Average %	95
	Range %	91-99
1:800	Average %	94
	Range %	88-98
1:1600	Average %	105
	Range %	101-109

回收率：

The recovery of human VLDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-95
EDTA plasma (n=4)	103	98-107

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.