Human type Ⅱ collagen helical peptide,HELIX-Ⅱ ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

人Ⅱ型胶原螺旋肽(HELIX-Ⅱ)酶联免疫试剂盒（CSB-E09708h）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液样本中的HELIXⅡ含量。试剂盒检测范围为1.25 ng/mL-80 ng/mL，Ⅱ型胶原是关节软骨的主要结构蛋白，其螺旋结构域（HELIX - Ⅱ）的降解产物可作为软骨代谢异常的分子标志物，在骨关节疾病机制研究、软骨损伤动态监测及药物干预效果评价等领域具有重要科研价值。研究人员可通过该产品探究骨关节炎、类风湿性关节炎等疾病进程中软骨基质降解的动态变化，或用于筛选调节软骨代谢的潜在化合物，为相关领域的分子机制研究和治疗策略开发提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

type 2 collagen helical peptide,HELIX-2,_x000D_ type II collagen helical peptide,HELIX-II
缩写：

HELIXⅡ
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, urine
检测范围：

1.25 ng/mL-80 ng/mL
灵敏度：

0.312 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human HELIX-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-99
1:2	Average %	89
	Range %	83-94
1:4	Average %	99
	Range %	94-103
1:8	Average %	104
	Range %	100-112

回收率：

The recovery of human HELIX-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	80-88
EDTA plasma (n=4)	91	86-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.