Human soluble amyloid precursor protein α (sAPPα) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

人可溶性淀粉酶前体蛋白α(sAPPα)酶联免疫试剂盒（CSB-EQ027464HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、组织培养上清液、脑脊液（CSF）样本中的sAPPα含量。sAPPα是一个备受关注的靶点。其它在生理过程中有重要作用。研究机制上，目前认为其与神经系统等功能相关，参与神经保护、突触可塑性调节等过程，深入探索其作用机制有望为相关神经疾病治疗带来新突破。试剂盒检测范围为12.5 ng/mL-800 ng/mL，适用于神经科学领域的基础研究，包括疾病模型中的蛋白代谢分析、细胞实验中的分泌动态监测，以及药物干预下sAPPα水平变化的评估等科研场景，为探索神经系统疾病机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

soluble amyloid precursor protein alpha, sAPP alpha
缩写：

sAPPα
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates, cell culture supernates, cerebrospinal fluid (CSF)
检测范围：

12.5 ng/mL-800 ng/mL
灵敏度：

3.12 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human sAPPα in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	82-94
1:2	Average %	101
	Range %	90-105
1:4	Average %	91
	Range %	85-94
1:8	Average %	93
	Range %	85-97

回收率：

The recovery of human sAPPα spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	85-97
EDTA plasma (n=4)	97	90-101

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.