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  4. Human pyridinoline (PYD) ELISA Kit

Human pyridinoline (PYD) ELISA Kit

文献引用 (1) 说明书下载
  • 中文名称：
    人胶原吡啶交联(PYD)酶联免疫试剂盒
  • 货号：
    CSB-E09702h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    人胶原吡啶交联(PYD)酶联免疫试剂盒（CSB-E09702h）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液样本中的PYD含量。试剂盒检测范围为12.5 ng/mL-800 ng/mL，适用于骨代谢机制研究、抗骨质疏松药物筛选、关节炎动物模型评估等科研领域。特别针对尿液样本的兼容性设计，为无创性骨代谢动态监测提供了便捷方案，可满足体外培养体系检测、疾病模型纵向观察等实验需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    N/A
  • 缩写：
    PYD
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, urine
  • 检测范围：
    12.5 ng/mL-800 ng/mL
  • 灵敏度：
    3.12 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human PYD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %94
    Range %88-100
    1:2Average %92
    Range %86-99
    1:4Average %87
    Range %80-94
    1:8Average %97
    Range %92-102
  • 回收率：
    The recovery of human PYD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9385-99
    EDTA plasma (n=4)9892-104
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    8002.501 2.450 2.476 2.343
    4001.934 2.009 1.972 1.839
    2001.295 1.301 1.298 1.165
    1000.718 0.775 0.747 0.614
    500.483 0.495 0.489 0.356
    250.331 0.351 0.341 0.208
    12.50.226 0.235 0.231 0.098
    00.136 0.129 0.133 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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