Human proteasome (prosome, macropain) subunit, beta type, 2 (PSMB2) ELISA kit

Human proteasome (prosome, macropain) subunit, beta type, 2 (PSMB2) ELISA kit

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中文名称：

人蛋白酶体（prosome，macropain）亚基，β型，2(PSMB2)酶联免疫试剂盒
货号：

CSB-E17836h
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

人蛋白酶体（prosome，macropain）亚基，β型，2(PSMB2)酶联免疫试剂盒（CSB-E17836h）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的PSMB2含量。PSMB2 是一个重要靶点。它在多种生理和病理过程中发挥作用，可能与细胞内蛋白降解、免疫调节等机制相关。对其研究有助于深入了解疾病发生发展的分子机制，为开发针对该靶点的药物，治疗相关疾病提供理论依据。试剂盒检测范围为25 pg/mL-1600 pg/mL，适用于基础科研中探究蛋白酶体功能调控机制、肿瘤发生发展相关蛋白代谢异常、神经退行性疾病病理模型中的蛋白稳态失衡等研究领域；可高效支持体外培养细胞、动物模型组织或体液样本中PSMB2的定量分析，为研究蛋白质降解通路在疾病发生中的作用或药物干预效果评价提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

HC7 I ELISA Kit; Macropain subunit C7 I ELISA Kit; Macropain subunit C7-I ELISA Kit; Multicatalytic endopeptidase complex subunit C7 1 ELISA Kit; Multicatalytic endopeptidase complex subunit C7 I ELISA Kit; Multicatalytic endopeptidase complex subunit C7-I ELISA Kit; Proteasome (prosome, macropain) subunit beta type 2 ELISA Kit; Proteasome beta 2 subunit ELISA Kit; Proteasome component C7 I ELISA Kit; Proteasome component C7-I ELISA Kit; Proteasome subunit beta type-2 ELISA Kit; PSB2_HUMAN ELISA Kit; Psmb2 ELISA Kit
缩写：

PSMB2
Uniprot No.：

P49721
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

25 pg/mL-1600 pg/mL
灵敏度：

6.2 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cell Biology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human PSMB2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	104
	Range %	99-108
1:2	Average %	86
	Range %	83-89
1:4	Average %	94
	Range %	89-98
1:8	Average %	93
	Range %	88-97

回收率：

The recovery of human PSMB2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	88	82-94

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1600	2.750	2.852	2.801	2.656
800	2.048	2.147	2.098	1.953
400	1.285	1.383	1.334	1.189
200	0.763	0.785	0.774	0.629
100	0.416	0.424	0.420	0.275
50	0.251	0.262	0.257	0.112
25	0.204	0.210	0.207	0.062
0	0.144	0.145	0.145

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Identification of a Novel Serum Biomarker for Tuberculosis Infection in Chinese HIV Patients by iTRAQ-Based Quantitative Proteomics Chen C,Frontiers in microbiology,2023

功能：

Non-catalytic component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).
基因功能参考文献：
1. Results showed that the overexpression of beta2 subunit decreased homologous recombination in human cells without altering the cell proteasome activity and the Rad51p level. PMID: 21660142
2. This subunit propeptides influence cooperative proteasome assembly. PMID: 12456675
亚细胞定位：

Cytoplasm. Nucleus.
蛋白家族：

Peptidase T1B family
数据库链接：

HGNC: 9539

OMIM: 602175

KEGG: hsa:5690

STRING: 9606.ENSP00000362334

UniGene: Hs.471441

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.