Human prostacycline,PGI2 ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO人前列环素（PGI2）酶联免疫检测试剂盒（货号：CSB-E09591h），采用竞争法原理实现定量分析，适用于检测血清、血浆样本中的PGI2水平。PGI2是由血管内皮细胞合成的重要生物活性物质，具有扩张血管和抑制血小板聚集的功能，其水平变化与心血管疾病、炎症反应及血栓形成等病理过程密切相关。本试剂盒通过待测样本中的PGI2与酶标记物竞争结合特异性抗体的特性，结合显色反应测定吸光度值，最终通过标准曲线精确计算目标物浓度，检测灵敏度覆盖50 pg/mL至2000 pg/mL的线性范围，可满足低丰度样本的检测需求。该产品适用于心血管机制研究、药物对血管内皮功能影响的体外评估，以及炎症或血栓性疾病模型中PGI2动态变化的定量分析，为探索血管调节机制、抗血小板药物开发等科研方向提供可靠工具。实验操作需严格按照说明书进行样本预处理（如避免反复冻融、离心去除颗粒物等），以确保检测结果的重复性和准确性。
缩写：

PGI2
种属：

Homo sapiens (Human)
样本类型：

serum, plasma
检测范围：

50 pg/mL-2000 pg/mL
灵敏度：

50 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Biochemicals
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human PGI2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	86-101
1:2	Average %	94
	Range %	86-100
1:4	Average %	94
	Range %	91-96
1:8	Average %	95
	Range %	85-105

回收率：

The recovery of human PGI2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	88-98
EDTA plasma (n=4)	96	92-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.