Human pro-prostate specific antigen (Pro-PSA) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<20%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

人前列腺特异性抗原前体(Pro-PSA)酶联免疫试剂盒（CSB-ET027786HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Pro-PSA含量。Pro-PSA即前列腺特异性抗原前体，在前列腺癌的诊断和病情评估中有重要意义。其研究机制主要是通过检测体内Pro-PSA含量，与总PSA等指标结合，能更准确地鉴别前列腺癌与良性前列腺疾病，提高早期诊断的准确性。试剂盒检测范围为125 pg/mL-4000 pg/mL，该产品适用于探索前列腺疾病分子机制、评估生物标志物表达特征、筛选潜在治疗靶点等基础研究，也可用于细胞模型或动物模型中Pro - PSA的动态监测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

Pro-PSA
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

125 pg/mL-4000 pg/mL
灵敏度：

62.5 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human Pro-PSA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	89-99
1:2	Average %	90
	Range %	84-94
1:4	Average %	105
	Range %	98-108
1:8	Average %	92
	Range %	88-96

回收率：

The recovery of human Pro-PSA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	83-94
EDTA plasma (n=4)	93	89-95

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Serum proPSA as a marker for reducing repeated prostate biopsy numbers Sema Nur Ayy谋ld谋z.et al,Turkish Journal of Biochemistry T眉rk Biyokimya Dergisi,2017