Human pro-gastrin-releasing peptide, ProGRP ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO人胃泌素释放肽前体（ProGRP）定量检测试剂盒（货号CSB-E09127h），采用双抗体夹心酶联免疫法原理，适用于检测血清、血浆及组织匀浆样本中的ProGRP含量。ProGRP是胃泌素释放肽的前体蛋白，其异常表达与神经内分泌肿瘤的发生发展密切相关，尤其在肺癌等恶性肿瘤研究中具有重要生物学意义。试剂盒检测灵敏度为18.75 pg/mL，线性范围为18.75-1200 pg/mL，可精准量化样本中低浓度至中高浓度的目标蛋白水平。实验操作通过预包被特异性抗体的微孔板捕获样本中的ProGRP，经酶标记抗体二次结合后显色，通过标准曲线实现定量分析。该试剂盒适用于肿瘤标志物筛选、疾病机制研究及抗肿瘤药物开发等科研领域，尤其为肺癌相关生物标志物探索、病理模型验证及组织微环境分析提供可靠工具，其优化的检测体系支持多样本类型的高通量检测需求，满足实验室基础研究与转化医学应用。
缩写：

ProGRP
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

18.75 pg/mL-1200 pg/mL
灵敏度：

4.68 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human ProGRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	87
	Range %	80-90
1:10	Average %	97
	Range %	92-101
1:20	Average %	98
	Range %	93-102
1:40	Average %	93
	Range %	86-96

回收率：

The recovery of human ProGRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-96
EDTA plasma (n=4)	97	91-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.