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  4. Human placenta lactogen,HPL ELISA Kit

Human placenta lactogen,HPL ELISA Kit

文献引用 (3) 说明书下载
  • 中文名称：
    人胎盘催乳素(HPL)酶联免疫试剂盒
  • 货号：
    CSB-E09665h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO人胎盘催乳素（Human Placental Lactogen, HPL）酶联免疫检测试剂盒（货号CSB-E09665h），采用双抗体夹心法定量检测血清或血浆样本中的HPL蛋白。HPL是由胎盘合体滋养层细胞分泌的肽类激素，在妊娠中后期显著升高，通过调节母体代谢平衡和促进胎儿生长发育发挥重要作用。本试剂盒检测范围为80-4000 ng/mL，灵敏度为20 ng/mL，可在3.5小时内完成检测流程，提供预包被96孔板及标准品、检测抗体、HRP标记链霉亲和素等核心组分。该产品适用于科研领域对妊娠期胎盘功能、胎儿生长调控机制的研究，也可用于妊娠相关疾病模型的激素水平分析，例如妊娠糖尿病、子痫前期等病理状态的分子机制探索。实验操作兼容常规酶标仪检测，样本处理需遵循离体生物样本规范，建议通过预实验优化样本稀释比例以确保读数在标准曲线线性范围内。
  • 别名：
    CSH1Chorionic somatomammotropin hormone 1 ELISA kit; Choriomammotropin ELISA kit; Lactogen ELISA kit; Placental lactogen ELISA kit; PL ELISA kit
  • 缩写：
    CSH1
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma
  • 检测范围：
    80 ng/mL-4000 ng/mL
  • 灵敏度：
    100 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human HPL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %91
    Range %85-100
    1:2Average %96
    Range %90-105
    1:4Average %97
    Range %92-105
    1:8Average %95
    Range %89-100
  • 回收率：
    The recovery of human HPL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-98
    EDTA plasma (n=4)9790-103
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    40002.028 2.067 2.048 2.002
    16001.434 1.457 1.446 1.400
    6000.767 0.785 0.776 0.730
    2000.395 0.413 0.404 0.358
    800.192 0.196 0.194 0.148
    00.045 0.047 0.046 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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CSH1 antibodies

靶点详情

  • 功能：
    Produced only during pregnancy and is involved in stimulating lactation, fetal growth and metabolism. Does not interact with GHR but only activates PRLR through zinc-induced dimerization.
  • 基因功能参考文献：
    1. FGF-2b and hPL-A are promising candidates for regenerative therapy in Diabetes Mellitus by inducing de-differentiation of stem cells modulating pivotal endocrine genes. PMID: 29068419
    2. Placental lactogen is expressed but is not translated into protein in breast cancer. PMID: 24475273
    3. These results indicate that placental lactogen induces CYP2E1 expression via PI3-kinase pathway in human hepatocytes. PMID: 24408518
    4. Expression of placental lactogen mRNA is increased in plasma of women with placenta previa and invasive placenta. PMID: 23744883
    5. altered expression of placenta lactogen appears not to be causal for betamethasone-induced fetal growth restriction in the human PMID: 23465880
    6. results are consistent with a pleiotropic effect of placental hGH/CSH genes at the maternal-fetal interface relating to the regulation of fetal growth and the risk of affected maternal metabolism. PMID: 22387044
    7. Data conclude that members of the C/EBP and Ets families can differentially modulate CS-Benh and CS-Aenh activity. PMID: 21737519
    8. hPL-A is involved in the regulation of pancreatic beta cells activity. PMID: 21765243
    9. Results strongly support a conformationally mediated obligate-ordered prolactin receptor binding for each of the three lactogenic hormones: prolactin, growth hormone, and placental lactogen. PMID: 20427283
    10. The human growth hormone/chorionic somatomammotropin at chromosome 17 is implicated in regulation of postnatal and intrauterine growth. PMID: 20233782
    11. Investigation of the GRB2, GRB7, and CSH1 genes as candidates for the Silver-Russell syndrome (SRS) on chromosome 17q. PMID: 11897833
    12. a role for CSH1 haploinsufficiency in the etiology of growth retardation PMID: 14642004
    13. These data implicate ETS-domain family members in the regulation of the CS locus in placenta and pituitary cells. PMID: 14673137
    14. We showed that intra-arterial infusion of human placental lactogen primarily decreased coronary, renal & iliac blood flow. Mechanism of this response was shown to be due to inhibition of vasodilatory beta2-adrenergic receptor-mediated effect. PMID: 16410683
    15. crystal structure of the free state of human PL has been determined to 2.0 A resolution showing the molecule possesses an overall structure similar to other long chain four-helix bundle cytokines PMID: 16546209
    16. The present findings indicate that the concentration of human placental lactogen (hPL) and human chorionic gonadotropin (beta hCG) mRNA is significantly higher in the cellular component of maternal blood samples than in the plasma component. PMID: 16950818
    17. A CSH1 deletion carrier with nearly complete hypomethylation at the ICR1 locus had the characteristic clinical signs of Silver Russel syndrome, ruling out a causal relationship. PMID: 17400796
    18. Placental lactogen is a novel ligand of stabilin-1. PMID: 18292525
    19. Three single nucleotide polymorphisms in CGB5 and CSH1 genes were genotyped for 844 offspring of the cases and controls. Maternal breast cancer risk did not significantly differ by the offspring's carrier status of the three SNPs. PMID: 19047151
    20. maternal smoking associated with a reduction in cord blood hPL PMID: 19290844

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Somatotropin/prolactin family
  • 数据库链接：

    HGNC: 2440

    OMIM: 150200

    KEGG: hsa:1442

    STRING: 9606.ENSP00000316416

    UniGene: Hs.654390

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |