Human phosphoglycerate mutase 2,PGAM2 ELISA Kit

BPG dependent PGAM 2 ELISA Kit; BPG-dependent PGAM 2 ELISA Kit; GSD10 ELISA Kit; MGC88743 ELISA Kit; Muscle specific phosphoglycerate mutase ELISA Kit; Muscle-specific phosphoglycerate mutase ELISA Kit; OTTHUMP00000207787 ELISA Kit; PGAM 2 ELISA Kit; PGAM M ELISA Kit; PGAM-M ELISA Kit; Pgam2 ELISA Kit; PGAM2_HUMAN ELISA Kit; PGAMM ELISA Kit; phosphoglycerate mutase 2 (muscle) ELISA Kit; Phosphoglycerate mutase 2 ELISA Kit; Phosphoglycerate mutase isozyme M ELISA Kit

Homo sapiens (Human)

serum, plasma

3.9 pg/mL

1-5h

50-100ul

450 nm

Metabolism

quantitative

Sandwich

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

To assess the linearity of the assay, samples were spiked with high concentrations of human PGAM2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		88
		Range %		82-94
1:2		Average %		102
		Range %		98-105
1:4		Average %		95
		Range %		89-99
1:8		Average %		98
		Range %		91-102

The recovery of human PGAM2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		94		89-98
EDTA plasma (n=4)		98		92-104

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected   1000 2.526 2.417 2.472 2.327   500 1.608 1.505 1.557 1.412   250 0.884 0.855 0.870 0.725   125 0.486 0.494 0.490 0.345   62.5 0.329 0.306 0.318 0.173   31.2 0.265 0.257 0.261 0.116   15.6 0.197 0.189 0.193 0.048   0 0.145 0.144 0.145

3-5 working days