Human oxidized low density lipoprotein antibody,OLAb ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

人氧化低密度脂蛋白抗体（OLAb）酶联免疫试剂盒（货号：CSB-E08797h）是一款基于夹心法原理设计的定量检测试剂盒，适用于血清或血浆样本中OLAb的精准分析，检测范围为47-3000 μU/mL。OLAb是人体针对氧化修饰低密度脂蛋白产生的自身抗体，其水平变化与动脉粥样硬化、心血管疾病等病理过程密切相关，常用于科研领域探索氧化应激与血管内皮损伤的分子机制。本试剂盒通过高特异性抗体对实现目标分子的捕获与检测，操作流程标准化，可在4.5小时内完成实验，配套提供预包被96孔板、标准品、检测抗体及显色系统，用户仅需自备酶标仪即可开展研究。该产品适用于心血管疾病模型构建、代谢异常相关研究或炎症反应机制分析等科研场景，为评估氧化脂蛋白在疾病发展中的作用提供可靠工具。试剂盒需在2-8℃保存，开封后建议1个月内使用以保证最佳性能。
缩写：

ox-LDL Ab
种属：

Homo sapiens (Human)
样本类型：

serum, plasma
检测范围：

47 μU/mL-3000 μU/mL
灵敏度：

11.75 μU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human OLAb in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	90
	Range %	87-98
1:2000	Average %	93
	Range %	91-99
1:4000	Average %	95
	Range %	90-102
1:8000	Average %	92
	Range %	86-98

回收率：

The recovery of human OLAb spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	86-98
EDTA plasma (n=4)	96	91-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.