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Human oxidized high density lipoprotein (Ox-HDL)ELISA Kit

  • 中文名称:
    人氧化高密度脂蛋白(Ox-HDL)酶联免疫试剂盒
  • 货号:
    CSB-E16552h
  • 规格:
    96T/48T
  • 价格:
    ¥3600/¥2500
  • 其他:

产品详情

  • 产品描述:
    人氧化高密度脂蛋白(Ox-HDL)酶联免疫试剂盒(CSB-E16552h)为双抗夹心法ELISA试剂盒,定量检测血清、血浆、尿液、组织匀浆样本中的Ox-HDL含量。Ox - HDL指氧化型高密度脂蛋白,HDL本具抗动脉粥样硬化等有益作用,但氧化后功能改变。研究机制上,Ox - HDL可通过多种途径影响细胞功能,比如促使巨噬细胞摄取脂质形成泡沫细胞,参与动脉粥样硬化等疾病的发生发展。试剂盒检测范围为31.25 pg/mL-2000 pg/mL,可灵活应用于基础医学研究领域,如探究氧化应激状态下脂蛋白代谢机制、评估抗氧化药物干预效果、建立疾病模型生物标志物分析体系等;满足细胞实验、动物模型及临床前研究中对Ox-HDL的精准定量需求本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 缩写:
    Ox-HDL
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, urine, tissue homogenates
  • 检测范围:
    31.25 pg/mL-2000 pg/mL
  • 灵敏度:
    7.81 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Others
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human Ox-HDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 90  
    Range % 80-98  
    1:200 Average % 96  
    Range % 91-100  
    1:400 Average % 94  
    Range % 90-105  
    1:800 Average % 92  
    Range % 86-98  
  • 回收率:
    The recovery of human Ox-HDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 80-100  
    EDTA plasma (n=4) 90 80-98  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    2000 2.813 2.722 2.768 2.607  
    1000 2.122 2.036 2.079 1.918  
    500 1.154 1.198 1.176 1.015  
    250 0.623 0.595 0.609 0.448  
    125 0.368 0.347 0.358 0.197  
    62.5 0.292 0.272 0.282 0.121  
    31.25 0.205 0.192 0.199 0.038  
    0 0.164 0.158 0.161    
  • 数据处理:
  • 货期:
    3-5 working days

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