Human osteonectin,ON ELISA Kit

Human osteonectin,ON ELISA Kit

产品详情

引用文献

产品评价

靶点详情

产品详情

引用文献

产品评价

相关产品

靶点详情

Most popular with customers

产品中心

信号通路

技术信息

联系信息

中文名称：

人骨粘连蛋白(ON)酶联免疫试剂盒
货号：

CSB-E08760h
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情
产品评价
引用文献
相关产品
靶点详情

产品描述：

CUSABIO人骨粘连蛋白（Osteonectin, ON）酶联免疫检测试剂盒（货号：CSB-E08760h），采用双抗体夹心法定量检测血清、血浆、唾液及组织匀浆样本中的ON蛋白浓度。骨粘连蛋白作为钙结合糖蛋白，在骨骼矿化、细胞外基质组装及组织修复中发挥重要作用，其表达水平与骨代谢异常、肿瘤微环境调控等研究领域密切相关。本试剂盒检测灵敏度达0.78 ng/mL，线性范围覆盖0.78-50 ng/mL，仅需20-100 μL样本即可完成检测。试剂盒包含预包被捕获抗体、HRP标记检测抗体、标准品及显色底物等核心组分，标准品经严格校准可溯源至国际参考物质，开盖后2-8℃保存有效期达30天，未开封试剂盒建议-20℃避光保存。适用于科研场景下探究骨发育、心血管钙化、肿瘤转移等生物学过程中ON蛋白的动态变化，尤其适合多类型生物样本的批量检测需求，操作流程标准化且重复性良好，满足实验室对骨基质相关蛋白的高通量定量分析要求。
别名：

and Kazal-like domains proteoglycan 2 ELISA Kit; CWCV ELISA Kit; FLJ97039 ELISA Kit; KIAA0275 ELISA Kit; SPARC/osteonectin ELISA Kit; Sparc/osteonectin cwcv and kazal like domains proteoglycan (testican) 2 ELISA Kit; Sparc/osteonectin cwcv and kazal like domains proteoglycan 2 ELISA Kit; SPOCK 2 ELISA Kit; Spock2 ELISA Kit; Testican 2 ELISA Kit; Testican-2 ELISA Kit; Testican2 ELISA Kit; TICN 2 ELISA Kit; TICN2 ELISA Kit; TICN2_HUMAN ELISA Kit
缩写：

SPOCK2
Uniprot No.：

Q92563
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, saliva, tissue homogenates
检测范围：

0.78 ng/mL-50 ng/mL
灵敏度：

0.195 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of Human ON in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	94
	Range %	87-98
1:40	Average %	96
	Range %	90-102
1:80	Average %	94
	Range %	88-98
1:160	Average %	97
	Range %	92-103

回收率：

The recovery of Human ON spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	94-102
EDTA plasma (n=4)	86	80-92

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
50	2.658	2.857	2.758	2.663
25	2.307	2.208	2.258	2.163
12.5	1.601	1.543	1.572	1.477
6.25	0.847	0.867	0.857	0.762
3.12	0.485	0.474	0.480	0.385
1.56	0.307	0.319	0.313	0.218
0.78	0.181	0.188	0.185	0.090
0	0.094	0.095	0.095

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Hypoxic preconditioning enhances cell hypoxia tolerance and correlated lncRNA and mRNA analysis DongWang.et al,Life Sciences,2018
Osteonectin as a screening marker for pancreatic cancer: A prospective study Angeliki Papapanagiotou.et al,/,2018
Uncemented Primary Total Hip Arthroplasty, Presentation of Pain, and Expression of Osteonectin Beraudi A et al,Artif Organs.?,2013

功能：

May participate in diverse steps of neurogenesis. Binds calcium.
基因功能参考文献：
1. candidate susceptibility gene for bronchopulmonary dysplasia; lung expression pattern indicates potential role in alveolarization PMID: 21836138
2. Neurons are a major source of all of the testican family members in the normal brain. Abundant distribution of testican 2 may contribute to glioma invasion by inactivating other testican family members including N-Tes, which all inhibit MT-MMPs. PMID: 12810672
3. purification and characterization of a recombinant testican-2 expressed in insect cells; the C1q subcomponent of complement component C1 was identified as a potential interacting partner of testican-2 PMID: 17950619
亚细胞定位：

Secreted, extracellular space, extracellular matrix.
组织特异性：

Highly expressed in brain. Also found in lung and testis.
数据库链接：

HGNC: 13564

OMIM: 607988

KEGG: hsa:9806

STRING: 9606.ENSP00000321108

UniGene: Hs.523009

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.