Human orphanin FQ/nociceptin,OFQ/N ELISA Kit

Human orphanin FQ/nociceptin,OFQ/N ELISA Kit

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中文名称：

人孤腓肽(OFQ/N)酶联免疫试剂盒
货号：

CSB-E09741h
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

人孤腓肽(OFQ/N)酶联免疫试剂盒（CSB-E09741h）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的PNOC含量。PNOC是一种靶点。它可能参与体内多种生理过程，其相关研究机制或是从神经调节、内分泌等方面入手。通过对PNOC进行深入研究，有望在神经、内分泌等相关疾病的治疗上挖掘新的药物靶点，为开发新疗法提供理论依据。试剂盒检测范围为4.69 pg/mL-300 pg/mL，适用于神经生物学、药理学等领域的基础研究，例如探索疼痛机制、精神疾病模型中的肽类表达变化，或评估细胞实验中OFQ/N的分泌动态。通过可靠的定量结果，为科研人员解析OFQ/N在生理及病理过程中的调控作用提供有力工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

N/OFQ ELISA Kit; N23K ELISA Kit; N23K/N27K ELISA Kit; Nociceptin, included ELISA Kit; nocistatin ELISA Kit; Npnc1 ELISA Kit; OFQ ELISA Kit; OFQ/N ELISA Kit; Orphanin FQ ELISA Kit; Orphanin FQ2 ELISA Kit; Pnoc ELISA Kit; PNOC_HUMAN ELISA Kit; ppN/OFQ ELISA Kit; PPNOC ELISA Kit; pre-pro-N/OFQ ELISA Kit; prepronociceptin ELISA Kit; pronociceptin ELISA Kit; Propronociceptin ELISA Kit
缩写：

OFQ/N
Uniprot No.：

Q13519
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, cell culture supernates
检测范围：

4.69 pg/mL-300 pg/mL
灵敏度：

1.17 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human OFQ/N in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
Range %	80-105
1:2	Average %	96
Range %	85-114
1:4	Average %	93
Range %	92-114
1:8	Average %	95
Range %	80-110

回收率：

The recovery of human OFQ/N spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	80-110
EDTA plasma (n=4)	95	90-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
300	2.614	2.621	2.618	2.475
150	1.882	1.887	1.885	1.742
75	1.325	1.330	1.328	1.185
37.5	0.771	0.774	0.773	0.630
18.75	0.439	0.441	0.440	0.297
9.38	0.268	0.284	0.276	0.133
4.69	0.236	0.252	0.244	0.101
0	0.138	0.148	0.143

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Ligand of the opioid receptor-like receptor OPRL1. It may act as a transmitter in the brain by modulating nociceptive and locomotor behavior. May be involved in neuronal differentiation and development.; Blocks nociceptin action in pain transmission by inhibiting nociceptin-induced hyperalgesia and allodynia.; Has potent analgesic activity.
基因功能参考文献：
1. found differences in Neonatal Abstinence Syndrome outcomes depending on PNOC and COMT SNP genotype. PMID: 27983768
2. The presence of the PNOC rs732636 A allele was associated with the worse Neonatal abstinence syndrome outcomes in infants. PMID: 26791055
3. Data suggest that nociceptin plays a crucial role in progress of meiosis during spermatogenesis. [REVIEW] PMID: 25677772
4. Data suggest that both nociceptin and nocistatin inhibit myometrial contractions and may play roles in perception of pain during delivery. [REVIEW] PMID: 25677774
5. REVIEW: intracellular localization and physiological role of nociceptin and nocistatin PMID: 23454174
6. Strong expression of PNOC was seen in neoplastic neurons of 7/7 brainstem gangliogliomas, but was significantly weaker in non-brainstem GGs. PMID: 22706982
7. The presence of Orphanin, Oxytocin, ANP, eNOS and iNOS proteins was identified in the human umbilical cord.[Orphanin] PMID: 21744319
8. A high expression of nociceptin in adenomyosis may be one of the casual factors of dysmenorrhea. The severity of dysmenorrhea is positively correlated with the expression of nociceptin in eutopic endometrial tissue. PMID: 21600132
9. chronic ischemic conditions of atherosclerotic origin are associated with significantly lower plasma nociceptin levels PMID: 21310194
10. PNOC expression level was 2.99 times higher in vitiligo involved skin when compared to healthy control skin. PMID: 20888736
11. Results showed that vigorous and prolonged hyperventilation changes plasma nociceptin/orphanin FQ levels due to the direct effects of hypocapnic alkalosis or to different sympatho-adrenergic system responses. PMID: 20100531
12. Peripheral blood levels are elevated in Wilson disease PMID: 12812047
13. results show that nociceptin/NociR is present and functional in human neutrophils, and the results identify a novel dialogue pathway between neural and immune tissues PMID: 12950177
14. The presence of two different forms of mature nocistatin (NST-17 and NST-30) and a possible N-terminal methionine cleaved NST-29 have been confirmed by both radioimmunoassay and mass spectrometry. PMID: 16043263
15. Two SNPs in PNOC showed marginal association with alcoholism and one with illicit drug dependence. PMID: 17910740
16. Peripheral blood mononuclear cells transcribe pre-pro-nociception/orphaninFQ. PMID: 18292431
17. Unstable angina is associated with a significant increase in circulating nociceptin/orphanin FQ levels unrelated to intervening transient myocardial ischemia or hemodynamic changes. PMID: 19560501
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亚细胞定位：

Secreted.
蛋白家族：

Opioid neuropeptide precursor family
组织特异性：

Predominantly expressed in the brain and spinal cord. Also expressed and secreted by peripheral blood neutrophils following degranulation.
数据库链接：

HGNC: 9163

OMIM: 601459

KEGG: hsa:5368

STRING: 9606.ENSP00000301908

UniGene: Hs.88218

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.