Human orosomucoid 2,ORM2 ELISA Kit

Human orosomucoid 2,ORM2 ELISA Kit

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中文名称：

人类粘蛋白2(ORM2)酶联免疫试剂盒
货号：

CSB-E11821h
规格：

96T/48T
价格：

￥3200/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO人类粘蛋白2（ORM2）特异性酶联免疫试剂盒（货号：CSB-E11821h），采用竞争法定量检测血清、血浆及组织匀浆样本中ORM2的浓度。ORM2是一种由肝脏合成的急性期反应蛋白，在炎症调节、免疫应答及代谢相关疾病中发挥重要作用，其表达水平变化常与感染、创伤及慢性炎症状态相关。该试剂盒检测范围为78-5000 ng/mL，最低检测限为78 ng/mL，适用于科研领域中对ORM2蛋白表达水平的定量分析，例如探索炎症相关疾病机制、评估代谢综合征生物标志物或研究肝脏功能异常模型中的蛋白动态变化。实验操作兼容常规ELISA流程，支持多种生物样本类型：血清/血浆样本可直接用于检测，组织样本需制备成匀浆液后进行分析。本试剂盒包含预包被特异性抗体的微孔板、标准品及检测所需全套试剂，为科研人员研究ORM2在病理生理过程中的调控作用提供灵敏可靠的工具。
别名：

ORM2 ELISA Kit; AGP2Alpha-1-acid glycoprotein 2 ELISA Kit; AGP 2 ELISA Kit; Orosomucoid-2 ELISA Kit; OMD 2 ELISA Kit
缩写：

ORM2
Uniprot No.：

P19652
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

78 ng/mL-5000 ng/mL
灵敏度：

19.5 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human ORM2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	98
	Range %	90-102
1:200	Average %	89
	Range %	83-94
1:400	Average %	101
	Range %	93-105
1:800	Average %	105
	Range %	97-110

回收率：

The recovery of human ORM2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	82-95
EDTA plasma (n=4)	94	90-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
5000	0.131	0.135	0.133
2500	0.389	0.401	0.395
1250	0.750	0.732	0.741
625	1.042	1.020	1.031
312	1.441	1.454	1.448
156	1.650	1.666	1.658
78	1.806	1.767	1.787
0	2.001	2.078	2.040

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

最新研究进展：

ORM2，全称为酸性糖蛋白，是一种血浆蛋白。最近的研究表明，ORM2在多种疾病中发挥着重要作用，包括炎症、心血管疾病和肝病等。ORM2参与了细胞凋亡、免疫反应和糖代谢等生物学过程。
功能：

Functions as transport protein in the blood stream. Binds various hydrophobic ligands in the interior of its beta-barrel domain. Also binds synthetic drugs and influences their distribution and availability. Appears to function in modulating the activity of the immune system during the acute-phase reaction.
基因功能参考文献：
1. the glycosylation change of alpha-1-acid glycoprotein (AGP) in hepatocellular carcinoma (HCC), cirrhosis and controls PMID: 28621608
2. Neural cell interactions are important for brain physiology and pathology. Particularly, the interaction between non-neuronal cells plays a central role in regulating brain inflammation, which is closely linked to many brain disorders. Here, we newly identified orosomucoid-2 (ORM2) as an endogenous protein that mediates such non-neuronal glial cell interactions PMID: 28193696
3. Low ORM2 expression is associated with metastasis in hepatocellular carcinoma. PMID: 25965830
4. Data suggest that human serum albumin (HSA) might serve as a carrier in delivering chitooligomers to target tissues than alpha-1-glycoprotein (AGP) which has pharmacological importance. PMID: 24359035
5. Alteration in expression of ORM2 suggests that ORM2 could be used as a potential biomarker in the diagnosis of colorectal cancer. PMID: 22363757
6. the sera of patients with acute inflammation demonstrated increased numbers of bi-antennary and alpha1,3-fucosylated N-glycan structures at each glycosylation site PMID: 15863355
7. The binding of coumarin enantiomers to ORM2 is studied. PMID: 16290938
8. Results describe the thermal unfolding and reversibility of temperature-induced changes in alpha(1)-acid glycoprotein. PMID: 16331959
9. The results indicate that, in accordance with prior expectations, the ORM2 variant is responsible for the acute-phase property of alpha-1 acid glycoprotein. PMID: 19018521
10. structure of the alpha1-acid glycoprotein (AGP), or orosomucoid (ORM), gene was investigated in a Ghanaian mother and her child, who shared an unusual variant, ORM1 S2(C), found by isoelectric focusing PMID: 11587070
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亚细胞定位：

Secreted.
蛋白家族：

Calycin superfamily, Lipocalin family
组织特异性：

Expressed by the liver and secreted in plasma.
数据库链接：

HGNC: 8499

OMIM: 138610

KEGG: hsa:5005

STRING: 9606.ENSP00000394936

UniGene: Hs.719954

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.