Human neutrophil peptide 1-3,HNP1-3 ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO人中性粒细胞防御素1-3（HNP1-3）酶联免疫检测试剂盒（货号：CSB-E11742h），专为科研领域定量分析血清、血浆及组织匀浆样本中的HNP1-3水平而设计。HNP1-3属于α-防御素家族成员，主要由中性粒细胞分泌，在先天免疫中发挥关键作用，其表达水平与感染、炎症反应及部分病理过程密切相关。该试剂盒基于双抗体夹心法原理开发，检测灵敏度为1.56 ng/mL，线性检测范围覆盖1.56-100 ng/mL，能够精准检测低浓度样本。适用于探索感染性疾病机制、免疫调控研究、炎症相关疾病模型建立等科研场景，例如脓毒症、类风湿性关节炎或肿瘤微环境中的免疫应答研究。实验操作中可兼容多种生物样本类型，配套标准品及预包被板条可确保检测的稳定性和重复性，为科研人员提供高效可靠的定量分析工具。本产品仅供科学研究使用，不适用于任何临床诊断用途。
缩写：

HNP1-3
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

1.56 ng/mL-100 ng/mL
灵敏度：

0.39 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human HNP1-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	92-102
1:2	Average %	85
	Range %	81-90
1:4	Average %	95
	Range %	87-99
1:8	Average %	100
	Range %	85-106

回收率：

The recovery of human HNP1-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-93
EDTA plasma (n=4)	95	87-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.