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Human midregional pro-atrial natriuretic peptide (MR-proANP) ELISA kit

  • 中文名称:
    人中部前心钠肽(MR-proANP)酶联免疫试剂盒
  • 货号:
    CSB-EQ028056HU
  • 规格:
    96T/48T
  • 价格:
    ¥3600/¥2500
  • 其他:

产品详情

  • 产品描述:
    人中部前心钠肽(MR-proANP)酶联免疫试剂盒(CSB-EQ028056HU)为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的MR-proANP含量。试剂盒检测范围为39 pmol/L-2500 pmol/L,该产品适用于心血管疾病机制研究、动物模型中心功能评估、药物干预效果监测等科研领域,为探索心力衰竭、心肌缺血等疾病的分子机制提供定量分析工具。试剂盒操作流程标准化,配套完整实验组分,支持实验室开展高通量样本检测,满足基础医学研究和生物标志物开发需求。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 缩写:
    MR-proANP
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    39 pmol/L-2500 pmol/L
  • 灵敏度:
    9.8 pmol/L
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Others
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human MR-proANP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 94  
    Range % 85-97  
    1:2 Average % 100  
    Range % 94-105  
    1:4 Average % 90  
    Range % 83-94  
    1:8 Average % 97  
    Range % 91-101  
  • 回收率:
    The recovery of human MR-proANP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-98  
    EDTA plasma (n=4) 102 92-107  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pmol/L OD1 OD2 Average Corrected  
    2500 2.155 1.988 2.072 1.926  
    1250 1.697 1.627 1.662 1.516  
    625 1.160 1.088 1.124 0.978  
    312 0.692 0.708 0.700 0.554  
    156 0.414 0.425 0.420 0.274  
    78 0.283 0.277 0.280 0.134  
    39 0.197 0.191 0.194 0.048  
    0 0.148 0.144 0.146    
  • 数据处理:
  • 货期:
    3-5 working days

产品评价

相关问答

 常见问题解答
Q:

我想知道MR-proANP和proANP或者NT-proANP有什么区别?

A:
ANP由心房分泌,由proANP前体分泌。MR-proANP和NT-proANP是两个不同的部分。心房利钠肽前体中的肽是MR-proANP。心房利钠肽前体n端肽是NT-proANP。MR是proANP的中间肽,NT是氨基(N)末端肽。