Human melanin-concentrating hormone (PMCH) ELISA kit

中文名称：

人黑色素浓缩激素(PMCH)酶联免疫试剂盒
货号：

CSB-EL018230HU
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

人黑色素浓缩激素(PMCH)酶联免疫试剂盒（CSB-EL018230HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PMCH含量。PMCH即前黑素聚集激素，它是一种神经肽。在其与能量代谢、睡眠觉醒等生理过程相关。研究机制上，主要是通过与特定受体结合，激活细胞内信号通路来发挥作用，目前在肥胖、睡眠障碍等病症的治疗研究中受关注。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于探索PMCH在能量代谢调控、神经内分泌系统中的作用机制，或用于评估疾病模型、药物干预实验中的PMCH动态变化。该产品严格适配科研场景，为代谢性疾病、神经科学等领域的基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

PMCH ELISA Kit; MCHPro-MCH [Cleaved into: Neuropeptide-glycine-glutamic acid ELISA Kit; NGE ELISA Kit; Neuropeptide G-E); Neuropeptide-glutamic acid-isoleucine ELISA Kit; NEI ELISA Kit; Neuropeptide E-I); Melanin-concentrating hormone ELISA Kit; MCH)] ELISA Kit
缩写：

PMCH
Uniprot No.：

P20382
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human PMCH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-100
1:2	Average %	87
	Range %	83-91
1:4	Average %	92
	Range %	88-96
1:8	Average %	90
	Range %	85-94

回收率：

The recovery of human PMCH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	95-104
EDTA plasma (n=4)	84	80-88

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.682	2.533	2.608	2.400
500	2.040	1.946	1.993	1.785
250	1.419	1.357	1.388	1.180
125	0.927	0.975	0.951	0.743
62.5	0.654	0.689	0.672	0.464
31.2	0.408	0.392	0.400	0.192
15.6	0.335	0.324	0.330	0.122
0	0.204	0.211	0.208

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

引用文献

Effects of mild cognitive impairment and sleep disorders on the minimum alveolar concentration value of sevoflurane L Wang, S Yang, Z Niu, Y Guo,BMC anesthesiology,2024

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功能：

MCH may act as a neurotransmitter or neuromodulator in a broad array of neuronal functions directed toward the regulation of goal-directed behavior, such as food intake, and general arousal. May also have a role in spermatocyte differentiation.
基因功能参考文献：
1. MCH is an anabolic neuropeptide with multiple and diverse physiological functions including a key role in energy homoeostasis; evidence suggests that, in the reproductive context, MCH is a modulator rather than a primary regulator. PMID: 23884861
2. Cerebrospinal fluid (CSF) levels were measured in 33 patients with Alzheimer's disease (AD) and 33 healthy subjects, to investigate the role of MCH and HCRT-1 in AD. PMID: 23667582
3. the changes in the levels of two hypothalamic neuropeptides, hypocretin-1 and melanin-concentrating hormone, measured in the human amygdala, are reported. PMID: 23462990
4. Melanin-concentrating hormone (MCH) neurons of human hypothalamus, as well as the MCH neurons of mouse hypothalamus, contain IL-6Ralpha. PMID: 22295972
5. PMCH mRNA expression level was increased 1.85 times (P < 0.01) in vitiligo uninvolved skin and 5.36 times (P < 0.01) in involved skin compared to healthy control skin. PMID: 20888736
6. Melanin-concentrating hormone is expressed and functional in human skin PMID: 12176038
7. RT-PCR analysis revealed a low expression of prepro-MCH and MCH receptor 1 (MCHR1) but not of MCHR2 transcript in tissular and peripheral blood immune cells PMID: 12220661
8. Review. Melanin-concentrating hormone (MCH) is a cyclic neuropeptide, which centrally regulates food intake and stress. MCH-R1 antagonists can probe additional functions of the peptide, both in the brain and in the peripheral nervous system. PMID: 12885269
9. Serum levels could serve as useful peripheral markers of changes in energy homeostasis. PMID: 15546902
10. hMCH increased water intake independent of food intake. It significantly increased both sucrose solution and food intake. PMID: 15870910
11. Single nucleotide polymorphisms in pro-melanin-concentrating hormone may be associated with obesity and a greater body mass index in olanzapine-treated schizophrenic patients PMID: 17541984
12. expression of PMCH by activated Th2 cells in vivo may directly link allergic inflammation to energy homeostasis and may contribute to the association between asthma and obesity PMID: 17640905
13. The total number of hypocretin-1 neurons was significantly reduced by 30% in HD brains (P = 0.015), while the total number of MCH neurons was not significantly altered (P = 0.100). PMID: 18498421
14. MCH has a role in inflammatory processes in the intestine PMID: 18650383
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亚细胞定位：

Secreted.
蛋白家族：

Melanin-concentrating hormone family
组织特异性：

Predominantly expressed in lateral hypothalamus, also detected in pallidum, neocortex and cerebellum. Also found in thymus, brown adipose tissue, duodenum and testis (spermatogonia, early spermatocytes and Sertoli cells). No expression in peripheral blood
数据库链接：

HGNC: 9109

OMIM: 176795

KEGG: hsa:5367

STRING: 9606.ENSP00000332225

UniGene: Hs.707990

Human melanin-concentrating hormone (PMCH) ELISA kit

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