Human melanin ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO人黑色素（Melanin）定量检测试剂盒，采用竞争法原理定量检测血清样本中靶标含量，适用于皮肤生理机制、色素代谢调控及黑色素相关疾病模型的科学研究。该试剂盒检测灵敏度达12.5 pg/mL，检测范围为12.5-1000 pg/mL，可覆盖血清样本中黑色素浓度的典型波动区间。通过预包被特异性抗体与样本中的黑色素竞争结合酶标抗原，结合TMB显色系统实现定量分析，操作流程包含样本处理、孵育、洗涤及读数步骤，全程约需4.5小时。产品提供预装96孔板、标准品、检测抗体及全套实验试剂，适用于体外培养模型、动物实验样本或人体血清中黑色素水平的动态监测，可支持黑色素生成调控机制、光老化研究、美白成分筛选等基础科研需求。实验需自备酶标仪（450nm主波长/630nm校正波长）及移液设备，建议对浓度超过检测上限的样本进行适当稀释后复测。
缩写：

melanin
种属：

Homo sapiens (Human)
样本类型：

serum
检测范围：

12.5 pg/mL-1000 pg/mL
灵敏度：

15.6 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human melanin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	89-102
1:2	Average %	95
	Range %	91-100
1:4	Average %	94
	Range %	92-97
1:8	Average %	95
	Range %	90-101

回收率：

The recovery of human melanin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-105
EDTA plasma (n=4)	96	91-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.