Human matrix extracellular phosphoglycoprotein,MEPE ELISA Kit

Human matrix extracellular phosphoglycoprotein,MEPE ELISA Kit

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中文名称：

人细胞外基质磷酸糖蛋白(MEPE)酶联免疫试剂盒
货号：

CSB-E09173h
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO人细胞外基质磷酸糖蛋白（MEPE）酶联免疫检测试剂盒（货号：CSB-E09173h），采用双抗体夹心法定量检测血清、血浆或组织匀浆样本中的MEPE蛋白，检测灵敏度为15.6 ng/mL，线性范围覆盖15.6-1000 ng/mL。MEPE是一种参与骨组织矿化调控的关键蛋白，在骨基质形成、磷酸盐代谢及骨重塑过程中发挥重要作用，其表达异常与骨质疏松、骨软化症等代谢性骨病相关。本试剂盒通过高特异性抗体对实现精准捕获与检测，实验过程中目标蛋白首先被预包被的捕获抗体固定，随后与标记抗体结合形成复合物，通过酶催化显色反应强度进行定量分析。适用于体外研究MEPE在骨代谢疾病模型中的表达调控、矿化相关信号通路机制探索，或评估不同处理条件下（如药物干预、基因修饰）生物样本中MEPE的浓度变化，为骨生物学、矿物质代谢等领域的科研工作提供可靠工具。
别名：

Matrix extracellular phosphoglycoprotein ELISA Kit; MEPE ELISA Kit; MEPE_HUMAN ELISA Kit; OF45 ELISA Kit; Osteoblast/osteocyte factor 45 ELISA Kit
缩写：

MEPE
Uniprot No.：

Q9NQ76
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 ng/mL-1000 ng/mL
灵敏度：

3.9 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human MEPE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	89
	Range %	84-93
1:10	Average %	98
	Range %	95-102
1:20	Average %	84
	Range %	80-88
1:40	Average %	90
	Range %	85-94

回收率：

The recovery of human MEPE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-102
EDTA plasma (n=4)	105	99-110

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
1000	2.603	2.769	2.686	2.516
500	1.967	2.075	2.021	1.851
250	1.290	1.194	1.242	1.072
125	0.631	0.679	0.655	0.485
62.5	0.476	0.506	0.491	0.321
31.2	0.341	0.357	0.349	0.179
15.6	0.254	0.246	0.250	0.080
0	0.170	0.169	0.170

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Promotes renal phosphate excretion and inhibits intestinal phosphate absorption. Promotes bone mineralization by osteoblasts and cartilage mineralization by chondrocytes. Regulates the mineralization of the extracellular matrix of the craniofacial complex, such as teeth, bone and cartilage. Promotes dental pulp stem cell proliferation and differentiation.
基因功能参考文献：
1. Results indicate an association of MEPE gene inactivation with decreased survival after DNA damage. PMID: 23570370
2. Mice deficient in an ortholog of MEPE show increased bone mass and are resistant to aging-related bone loss. PMID: 12421822
3. results indicated that MEPE appeared to play an important positive role in proliferation and osteogenesis differentiation of DPCs through interaction with downstream signals. PMID: 22341070
4. MEPE or the MEPE-derived acidic serine aspartate-rich MEPE-associated motif peptide may contribute to decreased bone mineralization in Oncogenic osteomalacia patients. PMID: 21739089
5. Among migraineurs with aura rs7698623 in MEPE (OR = 6.37; 95% CI 3.15-12.90; p = 2.7x10(-7)) and rs4975709 in IRX4 (OR = 5.06; 95% CI 2.66-9.62; p = 7.7x10(-7)) appeared to be associated with ischemic stroke. PMID: 21779381
6. Sclerostin acts through regulation of the PHEX/MEPE axis at the preosteocyte stage and serves as a master regulator of physiologic bone mineralization. PMID: 21312267
7. MEPE may play a regulatory role in periodontal ligament cell osteogenic differentiation. PMID: 19031789
8. These results suggest that abnormal MEPE cleavage occurs when PHEX activity is deficient in humans. PMID: 20581062
9. This study demonstrates the importance of posttranslational modification for the site-specific activity of MEPE and its ASARM peptide. PMID: 19998030
10. The patterns of expression of FGF-23, MEPE, and DMP1 differ markedly in trabecular bone, suggesting that individual osteocytes may have specialized functions. PMID: 19679205
11. MEPE/OF45, a new dentin/bone matrix protein and candidate gene for dentin diseases mapping to chromosome 4q21. Expressed during odontogenesis. PMID: 12489176
12. There is evidence for a hormone/enzyme/extracellular matrix protein cascade involving fibroblastic growth factor 23 (FGF23), a phosphate-regulating gene with homologies to (PHEX) and (MEPE)--REVIEW PMID: 12791601
13. MEPE is predominantly expressed by osteocytes in human bone, with significant expression by osteocytes within mineralized bone PMID: 15108058
14. The results are consistent with MEPE being involved in phosphate and bone metabolism in a normal population. PMID: 15292364
15. MEPE expression is higher when cells proliferate, whereas it is downregulated as cells differentiated PMID: 18547474
16. MEPE is a candidate phosphatonin involved in phosphate homeostasis, acting in both the kidney and the gastrointestinal tract PMID: 19005008
17. MEPE is expressed in salivary gland ducts. PMID: 15329369
18. MEPE is expressed on the luminal surface of kidney proximal tubules. PMID: 15954904
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亚细胞定位：

Secreted, extracellular space, extracellular matrix.
组织特异性：

Expressed by osteoblasts. Expressed by stem cells in dental pulp. Expressed by mesenchymal cells in dental papilla and dental pulp. Expressed in teeth, specifically in decidious dentin. Expressed in ondotoblasts. Expressed in salivary glands. Secreted fro
数据库链接：

HGNC: 13361

OMIM: 605912

KEGG: hsa:56955

STRING: 9606.ENSP00000354341

UniGene: Hs.676357

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.