Human macrophage inflammatory protein 5,MIP-5 ELISA Kit

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中文名称：

人巨噬细胞炎性蛋白5(MIP-5)酶联免疫试剂盒
货号：

CSB-E07997h
规格：

96T/48T
价格：

￥3200/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO人巨噬细胞炎性蛋白5（MIP-5/CCL15）酶联免疫检测试剂盒（货号：CSB-E07997h），采用双抗体夹心法原理实现定量检测，适用于血清、血浆及组织匀浆样本分析。MIP-5作为CC趋化因子家族成员，在炎症反应和免疫细胞趋化中发挥关键调控作用，其表达水平与自身免疫性疾病、感染性疾病及肿瘤微环境密切相关。试剂盒检测灵敏度达31.25 pg/mL，线性检测范围为31.25-2000 pg/mL，可精准检测生物样本中MIP-5的动态浓度变化。预包被高特异性捕获抗体与检测抗体协同工作，搭配优化的反应体系显色系统，确保检测结果稳定可靠。该产品为炎症机制研究、疾病模型评估及药物干预实验提供有效工具，特别适用于探索MIP-5在病理过程中的调控网络、验证体外细胞实验数据或评估动物模型治疗效果。配套提供标准品、预混试剂及操作流程说明，支持常规实验室开展定量检测，满足科研领域对趋化因子信号通路研究的实验需求。
别名：

C-C motif chemokine 15 ELISA Kit; C-C motif chemokine ligand 15 ELISA Kit; CC chemokine 3 ELISA Kit; CCL15 ELISA Kit; CCL15(29-92) ELISA Kit; CCL15_HUMAN ELISA Kit; Chemokine (C C motif) ligand 15 ELISA Kit; Chemokine CC-2 ELISA Kit; Chemokine CC2 ELISA Kit; HCC-2 ELISA Kit; HCC2 ELISA Kit; HMRP 2B ELISA Kit; HMRP2B ELISA Kit; Leukotactin 1 ELISA Kit; Leukotactin-1 ELISA Kit; LKN-1 ELISA Kit; LKN1 ELISA Kit; Macrophage inflammatory protein 5 ELISA Kit; MIP 1D ELISA Kit; MIP-1 delta ELISA Kit; MIP-1D ELISA Kit; MIP-5 ELISA Kit; MIP1 delta ELISA Kit; MIP1d ELISA Kit; MIP5 ELISA Kit; MRP 2B ELISA Kit; Mrp-2b ELISA Kit; Mrp2b ELISA Kit; NCC 3 ELISA Kit; NCC-3 ELISA Kit; NCC3 ELISA Kit; New CC chemokine 3 ELISA Kit; OTTHUMP00000163955 ELISA Kit; SCYA15 ELISA Kit; SCYL3 ELISA Kit; Small inducible cytokine A15 ELISA Kit; Small inducible cytokine subfamily A (Cys Cys); member 15 ELISA Kit; Small-inducible cytokine A15 ELISA Kit; SY15 ELISA Kit
缩写：

CCL15
Uniprot No.：

Q16663
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.81 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean(pg/ml)	244.575	250.280	257.432	237.463	249.328	256.000
SD	0.024	0.031	0.037	0.026	0.035	0.041
CV(%)	3.352	4.258	4.980	3.709	4.821	5.541

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human MIP-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:4

Average %

Range %

81-89

1:8

Average %

Range %

91-99

1:16

Average %

Range %

83-92

1:32

Average %

105

Range %

101-109

回收率：

The recovery of human MIP-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	95-103
Heparin plasma (n=4)	91	89-93

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
2000	2.652	2.701	2.677	2.505
1000	1.945	1.932	1.939	1.767
500	1.213	1.204	1.209	1.037
250	0.741	0.735	0.738	0.566
125	0.451	0.432	0.442	0.270
62.5	0.330	0.342	0.336	0.164
31.25	0.266	0.257	0.262	0.090
0	0.170	0.173	0.172

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

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功能：

Chemotactic factor that attracts T-cells and monocytes, but not neutrophils, eosinophils, or B-cells. Acts mainly via CC chemokine receptor CCR1. Also binds to CCR3. CCL15(22-92), CCL15(25-92) and CCL15(29-92) are more potent chemoattractants than the small-inducible cytokine A15.
基因功能参考文献：
1. CCL15 secreted from SMAD4-deficient xenografted human colorectal cancer cells recruited CCR1(+) cells, promoting their metastatic activities to the lung. Lung metastases from colorectal cancer patients revealed that CCL15 expression correlated with loss of SMAD4. CCL15-positive metastases recruited approximately 1.9 times more numbers of CCR1(+) cells than CCL15-negative metastases. PMID: 27492974
2. CCL15 activation of CCR1 plays critical roles in hepatocellular carcinoma metastasis. PMID: 26501423
3. Orthotopic animal models of HCC were established to investigate the role of CCL15 in hMSCs migration toward HCC in vivo. Both histological and flow cytometric analysis showed that significantly fewer hMSCs localized within 97H-CCL15-shRNA xenografts compared with 97H-green fluorescent protein xenografts after intravenous delivery PMID: 26763650
4. Loss of SMAD4 was significantly associated with CCL15 expression. PMID: 26341919
5. follicular thyroid carcinoma (FTC) might induce tumor associated macrophages (TAMs) infiltration by producing CCL15. Measurement of TAMs and CCL15 in follicular thyroid lesions may be applied clinically to differentiate FTC from follicular adenoma PMID: 26875556
6. two inflammatory cytokines, MIP-1delta and MIP-3alpha, are able to increase mesenchymal stromal cells migration in vitro PMID: 25579056
7. we investigated a role of CCL15 in upregulating ICAM-1 in endothelial cells PMID: 23690481
8. CCL15 is elevated in human asthma and correlates with disease severity . PMID: 22092970
9. Data show that Only three of these proteins EGF, PDGF-BB and MIP-1delta (CCL15) differed significantly in plasma between controls and Alzheimer's disease (AD). PMID: 22279551
10. increased secretion by macrophages during Mycobacterium tuberculosis infection PMID: 20016943
11. truncation of NH2-terminal amino acid residues increases agonistic potency on CC chemokine receptors 1 and 3 PMID: 11832479
12. Lkn-1 activates the ERK pathway by transducing the signal through G(i)/G(o) protein, PLC, PKC delta and Ras, and it may play a role for cell proliferation, differentiation, and regulation of gene expression for other cellular processes PMID: 12759139
13. These observations demonstrate that the two NF-kappaB binding sites are essential for phorbol myristate acetate-induced leukotactin-1 (Lkn-1)/CCL15 expression in human monocytes. PMID: 15179048
14. CCL15(25-92) has in vitro and in vivo angiogenic activity PMID: 15251437
15. alanine-aspartic acid residues preceding the first cysteine at the NH(2)-terminus are essential for the binding and biological activity of leukotactin-1 PMID: 15894113
16. Compared with full-length CCL15, proteolytically processed CCL15 isoforms with N-terminal deletions display increased potency to induce calcium fluxes and chemotactic activity on monocytes and to induce adhesiveness of mononuclear cells to fibronectin. PMID: 16034099
17. Transcription of the CCL15 gene is regulated by AP-1 and NF-kappaB through MEK and JNK MAPK pathways in monocytoid cells. PMID: 16364464
18. Results point to an involvement of the CCL15-CCR1 axis in the pathophysiology of chronic renal failure. PMID: 16737685
19. factor NF-kappaB plays an important role in regulation of LZIP expression, and LZIP expression regulates the monocyte cell migration induced by Lkn-1 PMID: 17296613
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亚细胞定位：

Secreted.
蛋白家族：

Intercrine beta (chemokine CC) family
组织特异性：

Most abundant in heart, skeletal muscle and adrenal gland. Lower levels in placenta, liver, pancreas and bone marrow. CCL15(22-92), CCL15(25-92) and CCL15(29-92) are found in high levels in synovial fluids from rheumatoid patients.
数据库链接：

HGNC: 10613

OMIM: 601393

KEGG: hsa:6359

STRING: 9606.ENSP00000432034

UniGene: Hs.272493

Human macrophage inflammatory protein 5,MIP-5 ELISA Kit

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