Human leukotriene E4,LT-E4 ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO定量检测人白三烯E4（LTE4）的酶联免疫试剂盒（货号：CSB-E05176h），适用于血清、血浆及组织匀浆样本分析。LTE4属于半胱氨酰白三烯家族，是花生四烯酸代谢通路的重要炎症介质，参与哮喘、过敏反应及慢性炎症等病理过程，其水平变化可反映炎症相关疾病的生物学进程。本试剂盒基于双抗体夹心法原理，检测范围为0.625-40 ng/mL，能够精准测定低浓度样本，满足多种实验需求。试剂盒提供预包被特异性抗体的微孔板，通过标准曲线实现目标物的定量分析，实验操作流程标准化，适用于炎症机制研究、药物筛选评估或疾病模型（如哮喘、过敏性鼻炎）中的生物标志物检测。科研人员可通过该产品探究LTE4在炎症信号通路中的作用，或评估抗炎药物对白三烯代谢的调控效果，为呼吸道疾病及免疫相关研究提供可靠工具。
缩写：

LT-E4
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.625 ng/mL-40 ng/mL
灵敏度：

0.156 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Biochemicals
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human LT-E4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	83-95
1:2	Average %	95
	Range %	91-98
1:4	Average %	95
	Range %	88-100
1:8	Average %	98
	Range %	91-103

回收率：

The recovery of human LT-E4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	81-93
EDTA plasma (n=4)	96	93-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.