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Human irisin ELISA kit

  • 中文名称:
    人鸢尾素酶联免疫试剂盒
  • 货号:
    CSB-EQ027943HU
  • 规格:
    96T/48T
  • 价格:
    ¥3600/¥2500
  • 其他:

产品详情

  • 产品描述:
    CUSABIO人鸢尾素(Irisin)定量检测试剂盒(货号:CSB-EQ027943HU),采用夹心法酶联免疫吸附原理,适用于科研领域中对血清、血浆及组织匀浆样本的精准检测。人鸢尾素是一种由运动诱导产生的肌细胞因子,在调节能量代谢、促进白色脂肪褐变等生理过程中发挥重要作用,其表达水平与肥胖、糖尿病等代谢性疾病的研究密切相关。该试剂盒检测灵敏度高,线性范围为3.12-200 ng/mL,可稳定识别天然及重组形式的人鸢尾素蛋白。实验操作全程无需特殊设备,配套试剂已完成优化预混,支持标准96孔板模式检测。科研人员可将其应用于运动生理学、代谢调控机制、脂肪组织功能研究等领域,通过定量分析不同干预条件下(如运动训练、药物处理)样本中鸢尾素浓度变化,为探索代谢相关疾病的分子机制提供可靠数据支持。本试剂盒检测特异性强,与啮齿类动物来源的鸢尾素无交叉反应,每批产品均通过严格的性能验证。
  • 缩写:
    irisin
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    3.12 ng/mL-200 ng/mL
  • 灵敏度:
    0.78 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Others
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human irisin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
      Sample Serum(n=4)    
    1:1 Average % 90    
    Range % 84-96    
    1:2 Average % 97    
    Range % 91-103    
    1:4 Average % 103    
    Range % 97-109    
    1:8 Average % 89    
    Range % 83-95    
  • 回收率:
    The recovery of human irisin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 100 94-106    
    EDTA plasma (n=4) 106 101-112    
                   
                   
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average Corrected    
    200 2.690 2.752 2.721 2.616    
    100 2.402 2.371 2.387 2.282    
    50 1.965 1.853 1.909 1.804    
    25 1.355 1.247 1.301 1.196    
    12.5 0.764 0.735 0.750 0.645    
    6.25 0.495 0.464 0.480 0.375    
    3.12 0.251 0.266 0.259 0.154    
    0 0.106 0.103 0.105      
  • 数据处理:
  • 货期:
    3-5 working days

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靶点详情

  • 最新研究进展:
    Irisin是一种由肌肉细胞产生的激素,在身体代谢调节中发挥着重要作用。最近的研究表明,Irisin对改善糖尿病、肥胖和心血管疾病等慢性病的预防和治疗具有潜在价值。此外,研究人员正在探索Irisin对其他疾病的影响,如阿尔茨海默病、肌肉萎缩和骨质疏松症等。虽然Irisin的作用机制还没有完全理解,但这种激素已经成为新的药物开发领域的热门研究方向之一。