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中文名称:人鸢尾素酶联免疫试剂盒
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货号:CSB-EQ027943HU
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规格:96T/48T
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价格:¥3600/¥2500
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其他:
产品详情
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产品描述:CUSABIO人鸢尾素(Irisin)定量检测试剂盒(货号:CSB-EQ027943HU),采用夹心法酶联免疫吸附原理,适用于科研领域中对血清、血浆及组织匀浆样本的精准检测。人鸢尾素是一种由运动诱导产生的肌细胞因子,在调节能量代谢、促进白色脂肪褐变等生理过程中发挥重要作用,其表达水平与肥胖、糖尿病等代谢性疾病的研究密切相关。该试剂盒检测灵敏度高,线性范围为3.12-200 ng/mL,可稳定识别天然及重组形式的人鸢尾素蛋白。实验操作全程无需特殊设备,配套试剂已完成优化预混,支持标准96孔板模式检测。科研人员可将其应用于运动生理学、代谢调控机制、脂肪组织功能研究等领域,通过定量分析不同干预条件下(如运动训练、药物处理)样本中鸢尾素浓度变化,为探索代谢相关疾病的分子机制提供可靠数据支持。本试剂盒检测特异性强,与啮齿类动物来源的鸢尾素无交叉反应,每批产品均通过严格的性能验证。
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缩写:irisin
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种属:Homo sapiens (Human)
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样本类型:serum, plasma, tissue homogenates
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检测范围:3.12 ng/mL-200 ng/mL
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灵敏度:0.78 ng/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Others
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测定原理:quantitative
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测定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human irisin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 90 Range % 84-96 1:2 Average % 97 Range % 91-103 1:4 Average % 103 Range % 97-109 1:8 Average % 89 Range % 83-95 -
回收率:
The recovery of human irisin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 100 94-106 EDTA plasma (n=4) 106 101-112 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 200 2.690 2.752 2.721 2.616 100 2.402 2.371 2.387 2.282 50 1.965 1.853 1.909 1.804 25 1.355 1.247 1.301 1.196 12.5 0.764 0.735 0.750 0.645 6.25 0.495 0.464 0.480 0.375 3.12 0.251 0.266 0.259 0.154 0 0.106 0.103 0.105 -
数据处理:
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货期:3-5 working days
引用文献
- Curcumin supplementation combined with high intensity interval training modulates serum irisin and lipid profile in obese women: "A randomized double-blind clinical trial" BJ Gharakhanlou, SB Bonab, A Amaghani,Contemporary Clinical Trials Communications,2025
- Acute high-intensity interval exercise is superior to moderate-intensity continuous exercise in enhancing endothelial function and its associated biomarkers in sedentary young individuals: the possible involvement of lactate Z Liu, J Huang, M Hu, X Cui, L Leng, K Wang,Journal of Exercise Science & Fitness,2024
- The impact of six-week dihydrogen-pyrroloquinoline quinone supplementation on mitochondrial biomarkers, brain metabolism, and cognition in elderly individuals with mild cognitive impairment: a randomized controlled trial S Baltic,Journal of Nutrition Health & Aging,2024
- تاثیر هشت هفته تمرین تناوبی شدید با مکملیاری جینکوبیلوبا بر تغییرات آیریزین و برخی شاخصهای التهاب و استرس مردان بیشوزن اسمعیلی,/,/
- Elevated serum irisin levels in boys with central precocious puberty independent of BMI D Zeng,Endokrynologia Polska,2024
- Serum Irisin in Polycystic Ovary Syndrome and its Alteration with Metformin Intervention R Telagareddy,/,2024
- Interleukin-15 Contributes to the Benefits of Βeta-Alanine Supplementation on the Performance of Elite Volleyball Players YR Qanbar,/,2024
- Comparative effects of different types of exercise on endothelial function, arterial stiffness, and executive function in sedentary young individuals: a randomized controlled trial J Huang,/,2024
- Serum irisin levels increase in girls with central precocious puberty not dependent on BMI: a pilot study Y Chen,Endocrine connections,2023
- The relationship between sarcopenia detected in newly diagnosed colorectal cancer patients and FGF21, irisin and CRP levels U Oflazoglu,European geriatric medicine,2023
- Relationship among Body Composition, Adipocytokines, and Irisin on Exercise Capacity and Quality of Life in COPD: A Pilot Study G Cuttitta,Biomolecules,2023
- HFpEF and Atrial Fibrillation: The Enigmatic Interplay of Dysmetabolism, Biomarkers, and Vascular Endothelial Dysfunction J Bosanac,Disease markers,2022
- Effects of functional training with blood occlusion on the irisin, follistatin, and myostatin myokines in elderly men F Pazokian,European Review of Aging and Physical Activity,2022
- The effects of different types of exercise on circulating irisin levels in healthy individuals and in people with overweight, metabolic syndrome and type 2 diabetes SG Parada-Sánchez,Physiological research,2022
- Overactivation of hepatic mechanistic target of rapamycin kinase complex 1 (mTORC1) is associated with low transcriptional activity of transcription factor EB and lysosomal dysfunction in dairy cows with clinical ketosis ZhiyuanFang,Journal of Dairy Science,2022
- Irisin levels are correlated with inflammatory markers in frontotemporal dementia VG Fraga,Journal of Clinical Neuroscience,2021
- Emerging evidence for the opposite role of circulating irisin levels and brown adipose tissue activity measured by infrared thermography in anthropometric and metabolic profile during childhood F De Meneck,Journal of Thermal Biology,2021
- Analysis of changes in serum levels and gene expression profiles of novel adipocytokines (Omentin, Vaspin, Irisin and Visfatin) and their correlation with serum C-reactive protein levels in women diagnosed with endometriosis E Kaya-Sezginer,/,2021
- Effect of Chronic Resistance Training on Circulating Irisin: Systematic Review and Meta-Analysis of Randomized Controlled Trials Pedro L. Cosio,International Journal of Environmental Research and Public Health,2021
- Effects of Interval Jump Rope Exercise Combined with Dark Chocolate Supplementation on Inflammatory Adipokine, Cytokine Concentrations, and Body Composition in Obese Adolescent Boys M Eskandari,Nutrients,2020
- Irisin level and neonatal birthweight: A systematic review and meta-analysis D Shan,European Journal of Obstetrics & Gynecology and Reproductive Biology,2020
- Several Circulating Biomarkers for PCOS Diagnosis Luo P, et al,Experimental and Clinical Endocrinology & Diabetes,2019
- Comprehensive evaluation of irisin levels in fetomaternal circulation of pregnant women with obesity or gestational diabetes mellitus Ali Seven,Irish Journal of Medical Science,2019
- Alpha lipoic acid protects against dexamethasone-induced metabolic abnormalities via APPL1 and PGC-1 α up regulation Mohammed MA, et al,Steroids,2019
- The effect of hypothyroidism on serum irisin level in patients with nondiabetic chronic kidney disease: A pilot study with a cross-sectional design Milind Machhindra Patil.et al,Saudi Journal of Kidney Diseases and Transplantation,2018
- Abnormal irisin level in serum and endometrium is associated with metabolic dysfunction in polycystic ovary syndrome patients Wei Wang.et al,CLIN ENDOCRINOL,2018
- The association between circulating irisin levels and different phenotypes of polycystic ovary syndrome L. Zhang.et al,Journal of Endocrinological Investigation,2018
- High Irisin Levels in Overweight/Obese Children and Its Positive Correlation with Metabolic Profile, Blood Pressure and Endothelial Progenitor Cells F De Meneck.et al,/,2018
- Irisin and Visfatin Predicts Severity of Diabetic Nephropathy Ramalingam Mageswari.et al,Indian Journal of Clinical Biochemistry,2018
- The combination of exercise training and Zataria multiflora supplementation increase serum irisin levels in postmenopausal women Ghanbari-Niaki, Abbass.et al,Integrative Medicine Research,2018
- SERUM IRISIN AND LEPTIN LEVELS IN OBESE AND NON-OBESE WOMEN WITH POLYCYSTIC OVARY SYNDROME WITH REFERENCE TO GLUCOSE SHATHA H. ALI.et al,International Journal of Pharmacy and Pharmaceutical Sciences ,2017
- Effects of physical activity on the link between PGC-1a and FNDC5 in muscle, circulating Ιrisin and UCP1 of white adipocytes in humans: A systematic review Dinas PC.et al,?F1000Res,2017
- Physiology and role of irisin in glucose homeostasis Nikolaos Perakakis Georgios A.et al,Nature Reviews Endocrinology,2017
- Free androgen index and Irisin in polycystic ovary syndrome Li H. et al,J Endocrinol Invest,2015
- Decreased saliva/serum irisin concentrations in the acute myocardial infarction promising for being a new candidate biomarker for diagnosis of this pathology. Aydin S.et al,Peptides.,2014
- Irisin levels before and after physical activity among school-age children with different BMI: A direct relation with leptin Palacios-González B et al,Obesity,2015
- Elevated Circulating Levels of Irisin and the Effect of Metformin Treatment in Women with Polycystic Ovary Syndrome Li M et al,J Clin Endocrinol Metab,2015
靶点详情
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最新研究进展:Irisin是一种由肌肉细胞产生的激素,在身体代谢调节中发挥着重要作用。最近的研究表明,Irisin对改善糖尿病、肥胖和心血管疾病等慢性病的预防和治疗具有潜在价值。此外,研究人员正在探索Irisin对其他疾病的影响,如阿尔茨海默病、肌肉萎缩和骨质疏松症等。虽然Irisin的作用机制还没有完全理解,但这种激素已经成为新的药物开发领域的热门研究方向之一。
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