Human intercellular adhesion molecule 3,ICAM-3 ELISA Kit

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中文名称：

人细胞间粘附分子3(ICAM-3/CD50)酶联免疫试剂盒
货号：

CSB-E07949h
规格：

96T/48T
价格：

￥3200/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO人细胞间粘附分子3（ICAM-3/CD50）酶联免疫试剂盒（货号CSB-E07949h），专为定量检测血清、血浆及组织匀浆样本中ICAM-3蛋白含量设计。ICAM-3作为免疫球蛋白超家族成员，主要在白细胞表面表达，参与免疫细胞间黏附及信号传导，在炎症反应、免疫应答调控及T细胞活化等生理过程中发挥重要作用。试剂盒采用双抗体夹心ELISA原理，通过高特异性配对抗体实现靶标捕获与检测，具备31.25-2000 pg/ml的宽泛检测范围，可精准量化低丰度样本中的目标蛋白。其优化的检测体系适用于多种生物样本类型，为研究免疫相关疾病机制、炎症模型建立、药物靶点验证等科研需求提供可靠工具，在自身免疫性疾病研究、感染免疫学实验及免疫调节剂开发等领域具有广泛应用潜力，实验操作全程无需复杂设备，满足实验室常规科研场景下的高效检测需求。
别名：

ICAM3; Intercellular adhesion molecule 3; ICAM-3; CDw50; ICAM-R; CD antigen CD50
缩写：

ICAM3
Uniprot No.：

P32942
种属：

Homo sapiens (Human)
样本类型：

serum, plasma,tissue homogenates
检测范围：

31.25 pg/ml - 2000 pg/ml
灵敏度：

7.8 pg/ml
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean(pg/ml)	225.927	236.470	246.378	218.347	231.580	242.642
SD	0.036	0.044	0.031	0.036	0.046	0.029
CV(%)	4.250	5.052	3.865	4.311	5.336	3.532

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human ICAM-3/CD50 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

89-100

1:2

Average %

Range %

84-92

1:4

Average %

103

Range %

98-107

1:8

Average %

Range %

89-97

回收率：

The recovery of human ICAM-3/CD50 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	80-88
EDTA plasma (n=4)	96	91-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
2000	2.752	2.654	2.703	2.548
1000	1.981	2.015	1.998	1.843
500	1.407	1.386	1.397	1.242
250	0.846	0.872	0.859	0.704
125	0.529	0.508	0.519	0.364
62.5	0.318	0.321	0.320	0.165
31.25	0.235	0.229	0.232	0.077
0	0.153	0.157	0.155

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

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最新研究进展：

ICAM3又称为白细胞共同抗原LFA-1，是一种细胞表面分子，属于免疫球蛋白超家族，主要在白细胞、淋巴细胞、巨噬细胞等免疫细胞表面表达。ICAM3参与调节免疫细胞之间的黏附作用，在细胞介导免疫应答、免疫细胞迁移和肿瘤免疫等方面发挥着重要作用。
功能：

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2. In association with integrin alpha-L/beta-2, contributes to apoptotic neutrophil phagocytosis by macrophages.
基因功能参考文献：
1. exploration of the underlying mechanism demonstrated that ICAM3 not only binds to LFA-1 with its extracellular domain and structure protein ERM but also to lamellipodia with its intracellular domain which causes a tension that pulls cells apart (metastasis). PMID: 29729315
2. Increased expression of PECAM-1, ICAM-3, and VCAM-1 in colonic biopsies from patients with inflammatory bowel disease (IBD) in clinical remission is associated with subsequent flares; this suggests that increases in the expression of these proteins may be early events that lead to flares in patients with IBD PMID: 27552332
3. ICAM3 acts as recognition receptors in the phagocytosis portals of macrophages for engulfment of apoptotic neutrophils. PMID: 23775590
4. this data clearly indicate that ICAM-3 promotes drug resistance via inhibition of apoptosis. PMID: 24177012
5. Intercellular adhesion molecule (ICAM)-3 mRNA is upregulated in non-adherent endothelial forming cells. PMID: 23144795
6. Results indicate that the ICAM-3 gene promoter is negatively regulated by RUNX3. PMID: 22479382
7. analysis of activated apoptotic cells induce dendritic cell maturation via engagement of Toll-like receptor 4 (TLR4), dendritic cell-specific intercellular adhesion molecule 3 (ICAM-3)-grabbing nonintegrin (DC-SIGN), and beta2 integrins PMID: 22396536
8. ICAM-3 may be an important adhesion molecule involved in chemotaxis to apoptotic human leukocytes. PMID: 22117198
9. the molecular basis of allergen-induced Th2 cell polarization PMID: 22205703
10. the cross-talk between neutrophils and NK cells is mediated by ICAM-3 and CD11d/CD18, respectively. PMID: 21712539
11. Single nucleotide polymorphisms in ICAM3 gene is associated with lymphoma. PMID: 21239057
12. induction of morphological polarization in primary T lymphocytes and Jurkat cells enhances Kidins220/ARMS colocalization with ICAM-3 PMID: 21381019
13. CCR1 antagonist, BX471, did not significantly alter ICAM-3 expression in relapsing-remitting multiple sclerosis patients. PMID: 20086017
14. ICAM-3 enhances the migratory and invasive potential of human non-small cell lung cancer cells by inducing MMP-2 and MMP-9 via Akt and CREB PMID: 19956847
15. no significant risk association was found for SARS infection for the ICAM-3 Asp143Gly SNP. PMID: 19801714
16. A novel serine-rich motif in the intercellular adhesion molecule 3 is critical for its ezrin/radixin/moesin-directed subcellular targeting PMID: 11784723
17. identification of DC-SIGN binding sites PMID: 11799126
18. interactions with DC-SIGN does not promote DC-SIGN mediated HIV-1 transmission PMID: 12021323
19. Expression of DC-SIGN and its ligand, ICAM-3, is found in substantial amounts only in RA synovium, suggesting that their interaction is implicated in the additional activation of synovial macrophages that leads to the production of EMMPRIN and MMP-1. PMID: 12571844
20. ICAM-3 is highly expressed on the surface of human eosinophils and has a role in the downregulation of GM-CSF production. PMID: 12743567
21. Relationship of intercellular adhesion molecule-3 and hepatocyte growth factor with amyloidosis A in chronic renal-failure patients. There was a higher density of intercellular adhesion molecule-3-positive cells in the patients with amyloidosis A. PMID: 14704632
22. ICAM-3 is expressed on human bone marrow endothelial cells and controls endothelial integrity via reactive oxygen species-dependent signaling. PMID: 14726630
23. acts as a costimulating molecule to increase HIV-1 transcription and viral replication, a process allowing productive infection of quiescent CD4+ T lymphocytes. PMID: 15163761
24. Expression of ICAM-3 can be used as a valuable biomarker to predict the radiation resistance in cervical cancer that occurs during radiotherapy. PMID: 15880373
25. The results suggest that ICAM-3 assists in the interaction of granulocytes with DC-SIGN of dendritic cells. PMID: 17145745
26. Patients with SARS homozygous for ICAM3 Gly143 showed significant association with higher lactate dehydrogenase levels and lower total white blood cell counts. PMID: 17570115
27. This is the first case of CD20 positive mycosis fungoides involving a lymph node to be reported in the literature. PMID: 18261116
28. extended alpha(L)beta(2) with an open headpiece is required for ICAM-3 adhesion PMID: 18354203
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亚细胞定位：

Membrane; Single-pass type I membrane protein.
蛋白家族：

Immunoglobulin superfamily, ICAM family
组织特异性：

Leukocytes.
数据库链接：

HGNC: 5346

OMIM: 146631

KEGG: hsa:3385

STRING: 9606.ENSP00000160262

UniGene: Hs.654563

Human intercellular adhesion molecule 3,ICAM-3 ELISA Kit

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