Human histatin 5 ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

人富组蛋白(histatin-5)是一种在唾液中高度表达的阳离子抗菌肽，具有广谱抗真菌及抗细菌活性，在口腔免疫防御和伤口愈合中发挥重要作用。本试剂盒（货号：CSB-E09935h）采用双抗体夹心法原理，定量检测血清、血浆或唾液样本中的histatin-5蛋白，检测范围为12.5-800 ng/mL，灵敏度达12.5 ng/mL，适用于研究口腔微生物感染、炎症反应调控或免疫调节机制等科研领域。试剂盒包含预包被特异性抗体的96孔板、标准品、生物素标记检测抗体、链霉亲和素-HRP及显色底物，操作流程包括样本孵育、洗涤、显色及终止反应，配套提供标准曲线生成方案。该产品适用于体外检测，可用于评估口腔疾病模型、抗菌肽功能研究或唾液成分分析，为探索口腔黏膜免疫及宿主-微生物互作提供可靠工具。
别名：

N/A
缩写：

histatin-5
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, saliva
检测范围：

12.5 ng/mL-800 ng/mL
灵敏度：

3.12 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Biochemicals
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human Histatin 5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	84-95
1:2	Average %	94
	Range %	90-98
1:4	Average %	100
	Range %	95-104
1:8	Average %	102
	Range %	96-108

回收率：

The recovery of human Histatin 5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	97-103
EDTA plasma (n=4)	95	91-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.