Human hepatocyte growth factor activator(HGFA) ELISA kit

Human hepatocyte growth factor activator(HGFA) ELISA kit

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中文名称：

人肝细胞生长因子激活物(HGFA)酶联免疫试剂盒
货号：

CSB-E13160h
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

人肝细胞生长因子激活物(HGFA)酶联免疫试剂盒（CSB-E13160h）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的HGFAC含量。HGFAC即肝细胞生长因子激活物前体，是一种丝氨酸蛋白酶前体，在肝脏合成后释放入血。其可被凝血酶等激活，进而激活肝细胞生长因子，在细胞生长、运动和形态发生等生理过程及肿瘤侵袭转移等病理过程有研究，是潜在治疗靶点。试剂盒检测范围为78 pg/mL-5000 pg/mL，本试剂盒适用于基础医学研究领域，例如在肝病模型、肿瘤微环境或创伤修复研究中动态监测HGFA的表达变化，也可用于评估药物干预对HGFA信号通路的影响，为相关疾病的分子机制探索及治疗策略开发提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

HGFAC ELISA Kit; Hepatocyte growth factor activator ELISA Kit; HGF activator ELISA Kit; HGFA ELISA Kit; EC 3.4.21.-) [Cleaved into: Hepatocyte growth factor activator short chain; Hepatocyte growth factor activator long chain] ELISA Kit
缩写：

HGFAC
Uniprot No.：

Q04756
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

78 pg/mL-5000 pg/mL
灵敏度：

19.5 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human HGFA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	81-89
1:2	Average %	95
	Range %	91-99
1:4	Average %	87
	Range %	82-93
1:8	Average %	105
	Range %	101-109

回收率：

The recovery of human HGFA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-91
EDTA plasma (n=4)	94	91-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
5000	2.117	2.148	2.133	2.025
2500	1.696	1.682	1.689	1.581
1250	1.215	1.268	1.242	1.134
625	0.834	0.816	0.825	0.717
312	0.502	0.536	0.519	0.411
156	0.265	0.258	0.262	0.154
78	0.168	0.171	0.170	0.062
0	0.106	0.109	0.108

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Plasma macrophage-stimulating protein and hepatocyte growth factor levels are associated with prostate cancer progression Sugie S. et al,Hum Cell,2015

功能：

Activates hepatocyte growth factor (HGF) by converting it from a single chain to a heterodimeric form.
基因功能参考文献：
1. the HGF-activator has a role in multiple myeloma cells' catalysis of hepatocyte growth factor (HGF) activation PMID: 15172968
2. HGF/MET signaling and aberrant HGF-activator expression is associated with diffuse large B-cell lymphoma PMID: 16189274
3. KLK4 and KLK5 activate pro-HGFA. PMID: 18221492
4. Results suggest that hepatocyte growth factor activator is a major serum activator of pro-macrophage-stimulating protein. PMID: 19456860
5. HAI-1 (serine peptidase inhibitor, Kunitz type 1) specifically traps active form of HGF activator on cellular surface and regulates HGF activation in pericellular microenvironment. PMID: 11013244
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亚细胞定位：

Secreted. Note=Secreted as an inactive single-chain precursor and is then activated to a heterodimeric form.
蛋白家族：

Peptidase S1 family
组织特异性：

Liver.
数据库链接：

HGNC: 4894

OMIM: 604552

KEGG: hsa:3083

STRING: 9606.ENSP00000372224

UniGene: Hs.104

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.