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中文名称：人粒细胞趋化蛋白-2(GCP-2/CXCL6)酶联免疫试剂盒
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货号：CSB-E09990h
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规格：96T/48T
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价格：￥3200/￥2500
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其他：
产品详情
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产品描述：人粒细胞趋化蛋白-2（GCP-2/CXCL6）ELISA试剂盒（CSB-E09990h）采用双抗体夹心法定量检测血清、血浆或组织匀浆样本中GCP-2/CXCL6的蛋白表达水平，其检测灵敏度范围为78-5000 pg/mL。GCP-2/CXCL6属于CXC趋化因子家族，通过与CXCR1/CXCR2受体结合参与中性粒细胞趋化、血管生成及炎症反应调控，在感染性疾病、肿瘤微环境形成及组织修复等病理生理过程中发挥重要作用。本试剂盒通过高特异性抗体对实现精准捕获和检测，适用于体外实验中对炎症机制研究、药物干预效果评估或疾病模型中趋化因子动态变化的定量分析，例如在细胞因子网络研究、免疫调节实验或炎症相关动物模型的组织样本检测中，为科研人员提供可靠的实验工具。该产品严格遵循科研用途设计，操作流程标准化，可满足基础研究中对生物样本内GCP-2/CXCL6表达水平的定量检测需求。
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别名：C X C motif chemokine 6 ELISA Kit; Chemokine (C X C motif) ligand 6 ELISA Kit; Chemokine alpha 3 ELISA Kit; CKA 3 ELISA Kit; CKA-3 ELISA Kit; CKA3 ELISA Kit; CXCL 6 ELISA Kit; CXCL6 ELISA Kit; CXCL6_HUMAN ELISA Kit; GCP 2 ELISA Kit; GCP-2 ELISA Kit; Granulocyte chemotactic protein 2 ELISA Kit; N-processed variant 3 ELISA Kit; SCYB6 ELISA Kit; Small inducible cytokine B6 ELISA Kit; Small inducible cytokine subfamily B (Cys X Cys) member 6 ELISA Kit; Small inducible cytokine subfamily B (Cys X Cys) member b ELISA Kit; Small-inducible cytokine B6 ELISA Kit
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缩写：
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Uniprot No.：
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种属：Homo sapiens (Human)
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样本类型：serum, plasma, tissue homogenates
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检测范围：78 pg/mL-5000 pg/mL
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灵敏度：19.5 pg/mL
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Immunology
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测定原理：quantitative
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测定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean(pg/ml)
624.092
617.035
632.238
637.984
631.667
633.154
SD
0.032
0.040
0.049
0.051
0.055
0.060
CV(%)
3.683
5.398
6.274
6.544
6.294
6.762
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线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human GCP-2/CXCL6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
84
Range %
81-88
1:2
Average %
92
Range %
89-96
1:4
Average %
102
Range %
98-105
1:8
Average %
99
Range %
95-104
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回收率：
The recovery of human GCP-2/CXCL6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
87
83-90
EDTA plasma (n=4)
100
95-105
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标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml
OD1
OD2
Average
Corrected
5000
2.750
2.842
2.796
2.658
2500
1.947
2.055
2.001
1.863
1250
1.201
1.280
1.241
1.103
625
0.763
0.798
0.781
0.643
312
0.461
0.433
0.447
0.309
156
0.286
0.293
0.290
0.152
78
0.233
0.216
0.225
0.087
0
0.170
0.180
0.175
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数据处理：
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货期：3-5 working days
引用文献
- Gut Microbial Metabolite Trimethylamine N-Oxide Aggravates Pulmonary W Lu,American journal of respiratory cell and molecular biology,2022
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靶点详情
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功能：Chemotactic for neutrophil granulocytes. Signals through binding and activation of its receptors (CXCR1 and CXCR2). In addition to its chemotactic and angiogenic properties, it has strong antibacterial activity against Gram-positive and Gram-negative bacteria (90-fold-higher when compared to CXCL5 and CXCL7).
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基因功能参考文献：
- CXCL6 could reduce the expression of miR-515-5p in NSCLC cells; MiR-515-5p acted as a tumor suppressor by targeting CXCL6 in non-small cell lung cancer cell cells. PMID: 29136957
- Study has provided the first report of fibroblast-derived CXCL12 enhancement of CXCL6 secretion in colon cancer cells, and of both CXCL12 and CXCL6 co-operatively regulating metastasis through the PI3K/Akt/mTOR signaling pathway. PMID: 28811711
- CXCL6 expression is upregulated by Fli1 deficiency in fibroblasts and endothelial cells, potentially contributing to the development of fibrosis and vasculopathy in the skin, lung, and heart of systemic sclerosis. PMID: 28507181
- CXCL6 level is high in the serum of chronic hepatitis B patients.CXCL6 promotes human hepatocyte proliferation through the CXCR1-NFkappaB pathway and inhibits collagen I secretion by hepatic stellate cells. PMID: 27032929
- The role of PITX2 in glaucoma may be mediated partly by regulating the expression of CXCL6 and BBS5 and thus affecting immune functions and intraocular pressure. PMID: 27520585
- The neutrophil-recruiting chemokine GCP-2/CXCL6 is expressed in cystic fibrosis airways and retains its functional properties after binding to extracellular DNA. PMID: 25993443
- HIF-1alpha promotes HCC progression and metastasis by upregulating CXCL6 transcription in HCC cells. PMID: 25323032
- Expression of the CXCL8, CXCL6 and CXCL1 genes are under the primary control of 1,25-dihydroxyvitamin D3 and its receptor. PMID: 24250750
- Data suggest that expression of CXCL6 in trophoblasts is up-regulated during pregnancy development/placentation; CXCL6 expression inhibits trophoblast cell migration and invasion by down-regulating activity of MMP2 (matrix metalloproteinase 2). PMID: 23814098
- Overexpression of GCP-2 in mesenchymal stem cells has the potential to enhance their angiogenic and survival properties. PMID: 22886775
- Studies show that functional blocking of GCP-2 inhibits tumor growth and metastases. PMID: 21236563
- The aim of this study was to investigate the role of the transcription factors, AP-1 and NF-kappaB, in IL-6 and CXCL8 regulation in Jurkat T-cells. PMID: 20507572
- The level of expression of granulocyte chemotactic protein 2 correlates with the severity of periodontitis and appears to act as a yet unrecognized functional adjunct to interleukin-8 in pathological gingival tissues. PMID: 18842116
- results suggest that granulocyte chemotactic protein-2 is an additional ELR(+)-CXC chemokine expressed in endometrial stromal cells PMID: 12524079
- induction in mesenchymal cells by interleukin-1beta and down-regulation by interferon-gamma PMID: 12533683
- Neutrophil recruitment to inflammatory sites is mediated by two related receptors: CXCR1 and CXCR2. Both receptors share two ligands, interleukin-8 (CXCL8) and GCP-2 (CXCL6). PMID: 12628493
- expression in inflamed intestinal tissue in Crohn's disease PMID: 15214047
- production of GCP-2 by endothelial cells within the tumor can contribute to tumor development through neovascularization due to endothelial cell chemotaxis and to tumor cell invasion and metastasis PMID: 15652347
- LDL lipoprotein subunit L5 induces human umbilical vein endothelial cells (HUVEC) to express CXCL6. PMID: 17022986
- These findings indicate that the equilibrium between angiostatic and angiogenic factors during inflammation and tumor progression is rather complex and differs depending on the chemokine, cell type, and stimulus. PMID: 17827342
- These observations suggest a role for CXCL6 in the innate immune response to microbial invasion of the amniotic cavity. PMID: 18782286
- Report gonadotropin-releasing hormone-regulated CXCL6 expression in human placentation. PMID: 19369450
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亚细胞定位：Secreted.
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蛋白家族：Intercrine alpha (chemokine CxC) family
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数据库链接：
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