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中文名称:人促性腺激素释放激素(GnRH)酶联免疫试剂盒
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货号:CSB-E06880h
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规格:96T/48T
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价格:¥3600/¥2500
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其他:
产品详情
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产品描述:CUSABIO人促性腺激素释放激素(GnRH)酶联免疫检测试剂盒(货号:CSB-E06880h),采用双抗体夹心法定量检测血清、血浆及组织匀浆样本中的GnRH浓度,检测灵敏度为2.5 pg/ml,线性范围为2.5-100 pg/ml。GnRH作为下丘脑分泌的关键神经肽,通过调控促黄体生成素(LH)和促卵泡激素(FSH)的释放,在生殖内分泌系统中发挥核心作用,其动态变化与性成熟、生殖周期及激素反馈调节密切相关。该试剂盒适用于生命科学领域的研究,例如探究生殖生理机制、评估内分泌干扰物的效应、分析动物模型生殖轴功能异常等场景。实验操作兼容常规ELISA流程,配套标准品及生物素标记检测抗体,通过显色反应实现目标物浓度与吸光度的定量关联,为生殖生物学、神经内分泌学及发育生物学等基础研究提供可靠工具。
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别名:GNRH1 ELISA Kit; GNRH ELISA Kit; GRH ELISA Kit; LHRHProgonadoliberin-1 ELISA Kit; Progonadoliberin I) [Cleaved into: Gonadoliberin-1 ELISA Kit; Gonadoliberin I ELISA Kit; Gonadorelin ELISA Kit; Gonadotropin-releasing hormone I ELISA Kit; GnRH-I ELISA Kit; Luliberin I ELISA Kit; Luteinizing hormone-releasing hormone I ELISA Kit; LH-RH I); GnRH-associated peptide 1 ELISA Kit; GnRH-associated peptide I)] ELISA Kit
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缩写:GNRH1
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Uniprot No.:
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种属:Homo sapiens (Human)
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样本类型:serum, plasma, tissue homogenates
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检测范围:2.5 pg/ml-100 pg/ml.
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灵敏度:0.625 pg/ml
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Signal Transduction
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测定原理:quantitative
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测定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
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线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human GnRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
92
Range %
86-98
1:2
Average %
97
Range %
92-106
1:4
Average %
85
Range %
81-88
1:8
Average %
89
Range %
83-96
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回收率:
The recovery of human GnRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
91
87-98
EDTA plasma (n=4)
93
88-99
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标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml
OD1
OD2
Average
Corrected
100
2.346
2.489
2.418
2.378
50
1.725
1.832
1.779
1.739
25
0.968
1.050
1.009
0.969
6.5
0.352
0.350
0.351
0.311
2.5
0.176
0.179
0.178
0.138
0
0.039
0.040
0.040
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数据处理:
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货期:3-5 working days
引用文献
- Electrochemical detection of Gonadotropin-releasing hormone agonists for uterine fibroids based on Poly (L-Serine)/Ag NPs/GO nanocomposite modified … Cong Zhou,International Journal of ELECTROCHEMICAL SCIENCE,2022
相关产品
靶点详情
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功能:Stimulates the secretion of gonadotropins; it stimulates the secretion of both luteinizing and follicle-stimulating hormones.
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基因功能参考文献:
- High expression of LHRH is associated with ovarian cancer. PMID: 27793127
- GNRH (and GNRHR) are expressed in trophoblast cell populations and fallopian tube epithelium at tubal ectopic pregnancy sites. PMID: 26920257
- High LHRH expression is associated with sarcomas of bone and soft tissue. PMID: 27639272
- mutations in the region encoding the decapeptide associated with complete Idiopathic hypogonadotropic hypogonadism PMID: 26595427
- GnRH regulates trophoblast invasion via RUNX2-mediated MMP2/MMP9 expression. PMID: 26660506
- analysis of information transfer via gonadotropin-releasing hormone receptors to extracellular signal-regulated kinase or nuclear factor of activated T-cells PMID: 26644469
- our results showed that GnRH participates in the self-renewal capacity and stemness maintenance of LCSLCs by upregulating the JNK signaling pathway, and GnRH may be useful as an alternative lung cancer stem-like cells therapy. PMID: 25955300
- Melatonin affects the secretion of GnRH, LH and testosterone, improves sperm quality thereby regulating the testicular development and male reproduction. (Review) PMID: 25916694
- Data suggest differences in regulation of expression of PEDF (up-regulation) vs. VEGF (down-regulation) in granulosa cells explain reduced risk of ovarian hyperstimulation syndrome due to ovulation induction using GnRH/GNRHR agonists rather than hCG. PMID: 26308290
- No abnormalities were found in the patient group for the PROKR2 and GNRH1genes. In addition, no genomic rearrangements were identified in the healthy control individuals for the described genes PMID: 24002956
- Haploinsufficiency of Dmxl2, encoding a synaptic protein, causes infertility associated with a loss of GnRH neurons in humans and mice. PMID: 25248098
- Data indicate that the gene expression pattern is profoundly different between human chorionic gonadotropin (hCG) and gonadotropin-releasing hormone (GnRH) agonist. PMID: 24603682
- GnRH agonists fail to increase Bax expression and do not potentiate the cytotoxic activity of docetaxel PMID: 24722580
- GnRH, through heterotopic expression of its receptor, may be a potential regulator of CYP11B2 expression levels in some cases of aldosterone-producing adenoma. PMID: 24472523
- This review will summarize the current understanding of the mechanisms by which PACAP modulates gonadotrope function, with a focus on interactions with GnRH. PMID: 24095645
- Current research efforts aim to discover the mechanisms responsible for the decoding of the GnRH pulse signal by the gonadotrope. PMID: 24056171
- GPR101 is a critical requirement for GnRH-(1-5) transactivation of EGFR in Ishikawa cells. PMID: 24264576
- This review summarizes the mechanisms used by glial cells to control GnRH neuronal ativity and secretion. PMID: 23735672
- R31C GNRH1 is the only missense mutation that was identified in a CpG islet in nine congenital hypogonadotropic hypogonadism subjects from four unrelated families, giving evidence for a putative ''hot spot''. PMID: 23936060
- GnRHI, GnRHII and GnRH receptor expression correlated significantly with poor prognosis in breast cancer patients PMID: 23287110
- AXL and MET crosstalk to promote gonadotropin releasing hormone (GnRH) neuronal cell migration and survival. PMID: 23648337
- Olfactory ensheathing cells form a microenvironment for migrating GnRH-1 neurons during development. PMID: 23404564
- TRH, LH-RH and substance P are not affected in Alzheimer disease and Down's syndrome PMID: 24010162
- Kisspeptin and GnRH pulse generation. PMID: 23550012
- Kisspeptin resets the GnRH pulse generator in men, but does not appear to do so in women PMID: 23550003
- Spontaneous GnRH release is detected in the median eminence of transgenic mice that produce diurnal variations in GnRH. PMID: 23077052
- The role of several class 1 cytokines in regulating GnRH neuronal development, GnRH secretion, and GnRH expression in this REVIEW. PMID: 22161498
- data demonstrate that GnRH transcription is repressed by AR via multiple sequences in GnRH-P, including three Oct-1 binding sites, and that this repression requires the complex interaction of several transcription factors PMID: 22074952
- molecular mechanisms involved in GnRH/GnRHR signaling at the maternal-fetal interface. [Review] PMID: 22024993
- Mutations in GNRH1 have been associated with both mild and severe forms of GnRH deficiency, and may work in combination with other gene mutations to produce GnRH-deficient phenotypes. PMID: 21722705
- [review] Existing evidence suggests that neural pathways originating in hindbrain and hypothalamic feeding nuclei transmit information concerning glucose availability to GnRH neurons, which integrate numerous physiological signals and metabolic cues. PMID: 21855365
- Data show that higher levels of GnRH IgM antibodies were detected in patients with IBS and dysmotility, but not organic GI diseases, compared with healthy controls. PMID: 21714833
- Assessment of CGB and GNRH1 expression level in cancer patients' blood may be useful for indicating metastatic spread of tumor cells. PMID: 21827674
- Sleep is associated with a significant decline in GnRH pulse frequency in both older and younger postmenopausal women PMID: 21646369
- Deletion of the cell-specific enhancer region of GnRH implicates the GnRH promoter in mediating pubertal development and periodic reproductive cycling. PMID: 21368045
- Lactation is an important physiological model of the integration of energy balance and reproduction, as it involves activation of potent appetitive neuropeptide systems coupled to a profound inhibition of pulsatile GnRH/LH secretion--REVIEW PMID: 20727862
- A large literature dealing with various stressors that regulate gonadotrophin-releasing hormone secretion in a variety of species provides evidence that stress modulates GnRH secretion by activating the corticotrophin-releasing factor system--REVIEW PMID: 20727865
- The sources of glutamatergic inputs to the GnRH neurons are only just beginning to be examined and include the anteroventral periventricular nucleus as well as the possibility that GnRH neurons--REVIEW PMID: 20807514
- We then discuss possible neurobiological mechanisms underlying GnRH pulse generation, and conclude by proposing that kisspeptin neurons in the arcuate nucleus are key players--REVIEW PMID: 20951683
- homozygous inactivating mutations in the GNRH1 gene causing hypothalamic hypogonadotropic hypogonadism in patients were reported, validating definitively the pivotal role of GnRH in human pubertal development and reproduction--REVIEW PMID: 20887715
- GNRH1 mutations might cause congenital idiopathic (or isolated) hypogonadotropic hypogonadism (Review) PMID: 20389089
- The role of GnRH-GnRHR signaling at the maternal-fetal interface therefore appears to be limited to the regulation of trophoblast hCG production. PMID: 20400076
- Data describe the distribution and sex dimorphism of kisspeptin-immunoreactive elements in hypothalami, reveal contacts between kisspeptin-immunoreactive fibers and GnRH cells, and colocalize kisspeptins and neurokinin B in the infundibular nucleus. PMID: 20529119
- Metabolic signals are integrated at the levels of first-order neurons equipped with the proper receptors, ant that these neurons send their signals towards hypothalamic GnRH neurons which constitute the integrative element of this network. PMID: 20138117
- The major genes associated with GnRH-dependent pubertal disorders, are reviewed. PMID: 20188792
- Review: Using human GnRH deficiency as a paradigm and presenting original data from the screening of numerous candidate genes, we discuss the emerging model of patient-focused clinical genetic research. PMID: 20484732
- cadherin switching and p120(ctn) signaling as important targets of GnRH function and as novel mediators of invasiveness and tumor progression in ovarian cancer. PMID: 20118984
- data indicate that the promoter region between -992 and -795 contains elements both essential and sufficient for targeting gene expression to GnRH neurons PMID: 11875100
- Coupling of GnRH concentration and the GnRH receptor-activated gene program PMID: 12040003
- JunD activated by LHRH acts as a modulator of cell proliferation and cooperates with the anti-apoptotic and anti-mitogenic functions of LHRH. PMID: 12054733
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相关疾病:Hypogonadotropic hypogonadism 12 with or without anosmia (HH12)
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亚细胞定位:Secreted.
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蛋白家族:GnRH family
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