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  4. Human glypican 5 (GPC5) ELISA kit

Human glypican 5 (GPC5) ELISA kit

文献引用 (1) 说明书下载
  • 中文名称：
    人磷脂酰肌醇蛋白聚糖5(GPC5)酶联免疫试剂盒
  • 货号：
    CSB-EL009707HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    人磷脂酰肌醇蛋白聚糖5(GPC5)酶联免疫试剂盒（CSB-EL009707HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、腹水样本中的GPC5含量。GPC5 即磷脂酰肌醇蛋白聚糖 -5，是定位于细胞膜表面的糖蛋白。在研究机制上，它可调控细胞信号传导、细胞增殖与迁移。在多种癌症中异常表达，有望成抗癌治疗靶点，但相关研究还需深入，以充分明确其作用和应用潜力。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于基础医学研究中GPC5蛋白表达的动态监测，例如肿瘤微环境研究、神经发育机制探索或代谢性疾病模型中GPC5的功能分析，为体外实验样本的蛋白水平检测提供可靠工具，所有应用仅限于科学研究领域。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    bA93M14.1 ELISA Kit; glypican 5 ELISA Kit; Glypican 5 precursor ELISA Kit; glypican proteoglycan 5 ELISA Kit; Glypican-5 ELISA Kit; GPC5 ELISA Kit; GPC5_HUMAN ELISA Kit; Secreted glypican-5 ELISA Kit
  • 缩写：
    GPC5
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, ascitic fluid
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human GPC5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 93
    Range % 88-97
    1:2 Average % 97
    Range % 92-102
    1:4 Average % 100
    Range % 94-107
    1:8 Average % 108
    Range % 103-114
  • 回收率：
    The recovery of human GPC5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 102 95-106
    EDTA plasma (n=4) 95 89-104
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    20 2.051 2.012 2.032 1.847
    10 1.788 1.798 1.793 1.608
    5 1.374 1.368 1.371 1.186
    2.5 0.842 0.856 0.849 0.664
    1.25 0.494 0.485 0.490 0.305
    0.625 0.315 0.326 0.321 0.136
    0.312 0.279 0.281 0.280 0.095
    0 0.180 0.190 0.185 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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GPC5 antibodies

GPC5 Proteins

靶点详情

  • 功能：
    Cell surface proteoglycan that bears heparan sulfate.
  • 基因功能参考文献：
    1. Our results showed that GPC5 was lowly expressed in PCa cell lines. PMID: 28893348
    2. Taken together, our results suggest GPC5 as a tumor suppressor in PDAC and its expression is possibly regulated by miR-4295. Our study indicates that the miR-4295/GPC5 axis may play an important role in the pathogenesis of PADC and has potential applications for the development of PDAC therapy. PMID: 29407175
    3. Suggest that GPC5 is able to suppress the lung adenocarcinoma metastasis by competitively binding to Wnt3a and inactivating the Wnt/beta-catenin signaling pathway. PMID: 27806326
    4. GPC5 is a novel epigenetically silenced tumor suppressor, which inhibits tumor growth by suppressing Wnt/beta-catenin signaling in lung adenocarcinoma. PMID: 27157618
    5. GPC5 protein expression showed a close correlation with the tumorigenesis and tumor progression of prostate cancer, and that might be applied as a novel biomarker for the prediction of diagnosis and prognosis of prostate cancer. PMID: 26631038
    6. These results suggest that miR-297 acted as an oncogenic miRNA, partly by targeting GPC5, adenocarcinoma of the lung. PMID: 27554041
    7. Results suggest a potential association between CRP and GPC5 variants with lung cancer risk; variation in GKN1 is associated with chemotherapy response in the Chinese Han population. PMID: 25999661
    8. miR-709 may positively regulate invasion and metastasis of hepatocellular carcinoma through targeting GPC5. PMID: 25818666
    9. gene variants in IRF5, IRF8 and GPC5 were not associated with risk of relapse or disease progression in multiple sclerosis PMID: 24943672
    10. GPC5, a tumor suppressor, is regulated by miR-620 in lung adenocarcinoma. PMID: 24682381
    11. Variants in the 50-upstream region of GPC5 confer risk of lung cancer in never smokers PMID: 24456789
    12. These results indicate that GPC5 polymorphisms would be useful genetic indicators for Inflammatory demyelinating diseasess, including neuromyelitis optica and multiple sclerosis . PMID: 24135429
    13. The IL7R, TNFRSF1A, and GPC5 polymorphisms tended to be associated with having a second event of Multiple sclerosis within a year. PMID: 24130709
    14. Shh binds to HSPG co-receptors containing a glypican 5 core and 2-O-sulfo-iduronic acid to promote neural precursor proliferation. PMID: 23867465
    15. Data suggest that glypican-5 (GPC5) is a metastasis suppressor gene in non-small cell lung cancer (NSCLC) and may be a potential biomarker that predicts NSCLC metastasis. PMID: 23962560
    16. GPC5 is a gene required for normal neural tube development. PMID: 23223018
    17. findings suggested that SNP rs2352028 in GPC5 might confer a slight risk to lung cancer/adenocarcinoma PMID: 22236185
    18. GPC5 stimulates Hh signaling by facilitating/stabilizing the interaction between Hh and Ptc1. PMID: 21339334
    19. GPC5 is a novel genetic locus protective against sudden cardiac arrest PMID: 20360844
    20. This study supports that multiple sclerosis susceptibility at 13q31-32 may localize to the Glypican-5 gene. PMID: 20692050
    21. No association between GPC5rs2352028 variant and lung cancer in never smokers in meta-analysis of 7 genome-wide association studies under the random effects model. PMID: 20688270
    22. GPC5 polymorphism associated with susceptibility to lung cancer in never smokers PMID: 20304703
    23. GPC5 is a possible target for the 13q31-q32 amplification detected in lymphoma cell lines. PMID: 12721791
    24. Independent replication confirms GPC5 as a genetic risk factor for multiple sclerosis. PMID: 19010793
    25. ZIC2, SPRY2, and GPC5 genes are candidate genes suspected to explain the malformations associated with cerebral anomalies in the hypothesis of a contiguous gene syndrome in 13q deletion syndrome PMID: 19022413
    26. Confirm the association of polymorphisms within GPC5 with response to interferon-beta therapy in patients with multiple sclerosis. PMID: 19556317
    27. Genome-wide association study of gene-disease association and pharmacogenomic / toxicogenomic. (HuGE Navigator) PMID: 18195134

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  • 亚细胞定位：
    Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side.; [Secreted glypican-5]: Secreted, extracellular space.
  • 蛋白家族：
    Glypican family
  • 组织特异性：
    In adult, primarily expressed in the brain. Also detected in fetal brain, lung and liver.
  • 数据库链接：

    HGNC: 4453

    OMIM: 602446

    KEGG: hsa:2262

    STRING: 9606.ENSP00000366267

    UniGene: Hs.655675

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