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  4. Human glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) (GOT2) ELISA kit

Human glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) (GOT2) ELISA kit

文献引用 (3) 说明书下载
  • 中文名称：
    人谷氨酸谷草转氨酶2，线粒体（谷草转氨酶2）(GOT2)酶联免疫试剂盒
  • 货号：
    CSB-E17834h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO人谷氨酸谷草转氨酶2（GOT2）酶联免疫检测试剂盒（货号：CSB-E17834h），采用双抗体夹心法（Sandwich ELISA）实现定量分析，适用于检测血清、血浆及组织匀浆样本中GOT2的活性，检测范围为0.156-10 mU/mL，最低检测限达0.156 mU/mL。GOT2是线粒体内催化天冬氨酸与α-酮戊二酸转氨基反应的关键酶，参与细胞能量代谢和氧化还原平衡调节，其表达水平变化与线粒体功能异常、肝脏代谢调控及细胞应激响应等研究密切相关。本试剂盒通过高特异性抗体对靶标进行捕获与检测，确保数据的准确性和重复性，可为科研人员探究肝脏疾病模型、代谢通路调控、药物作用机制等基础研究提供可靠工具，尤其适用于动物实验或体外培养细胞中GOT2活性动态变化的定量分析，为揭示线粒体相关分子机制提供技术支持。
  • 别名：
    AATM_HUMAN ELISA Kit; AL022787 ELISA Kit; Aspartate aminotransferase 2 ELISA Kit; Aspartate aminotransferase ELISA Kit; Aspartate aminotransferase; mitochondrial ELISA Kit; Aspartate transaminase 2 ELISA Kit; ASPATA ELISA Kit; EC 2.6.1.1 ELISA Kit; FABP 1 ELISA Kit; FABP pm ELISA Kit; FABP-1 ELISA Kit; FABPpm ELISA Kit; Fatty acid binding protein ELISA Kit; Fatty acid-binding protein ELISA Kit; FLJ40994 ELISA Kit; Glutamate oxaloacetate transaminase 2 ELISA Kit; Glutamate oxaloacetate transaminase 2; mitochondrial ELISA Kit; Glutamate oxaloacetate transaminase; mitochondrial ELISA Kit; Glutamic oxaloacetic transaminase 2; mitochondrial (aspartate aminotransferase 2) ELISA Kit; Got 2 ELISA Kit; GOT2 ELISA Kit; KAT4 ELISA Kit; KATIV ELISA Kit; kynurenine aminotransferase 4 ELISA Kit; Kynurenine aminotransferase IV ELISA Kit; kynurenine--oxoglutarate transaminase 4 ELISA Kit; kynurenine--oxoglutarate transaminase IV ELISA Kit; mAspAT ELISA Kit; MGC102129 ELISA Kit; MGC115763 ELISA Kit; mitAAT ELISA Kit; mitochondrial ELISA Kit; Mitochondrial aspartate aminotransferase ELISA Kit; OTTMUSP00000017748 ELISA Kit; Plasma membrane fatty acid binding protein ELISA Kit; Plasma membrane-associated fatty acid-binding protein ELISA Kit; Transaminase A ELISA Kit
  • 缩写：
    GOT2
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 mU/mL-10 mU/mL
  • 灵敏度：
    0.039 mU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human GOT2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 85
    Range % 80-89
    1:2 Average % 92
    Range % 88-97
    1:4 Average % 96
    Range % 91-100
    1:8 Average % 87
    Range % 82-91
  • 回收率：
    The recovery of human GOT2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 103 98-109
    EDTA plasma (n=4) 95 90-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mU/ml OD1 OD2 Average Corrected
    10 2.186 2.268 2.227 2.028
    5 1.765 1.794 1.780 1.581
    2.5 1.391 1.380 1.386 1.187
    1.25 0.928 0.911 0.920 0.721
    0.625 0.654 0.623 0.639 0.440
    0.312 0.439 0.459 0.449 0.250
    0.156 0.327 0.341 0.334 0.135
    0 0.202 0.195 0.199 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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GOT2 antibodies

GOT2 Proteins

靶点详情

  • 功能：
    Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). As a member of the malate-aspartate shuttle, it has a key role in the intracellular NAD(H) redox balance. Is important for metabolite exchange between mitochondria and cytosol, and for amino acid metabolism. Facilitates cellular uptake of long-chain free fatty acids.
  • 基因功能参考文献：
    1. Study utilizing integrative analysis of transcriptomic, metabolomic, and clinical data propose a model of GOT2 transcriptional regulation, in which the cooperative phosphorylation of STAT3 and direct joint binding of STAT3 and p65/NF-kappaB to the proximal GOT2 promoter are important. Furthermore, high aberrant GOT2 expression is prognostic in diffuse large B-cell lymphoma. PMID: 29666362
    2. The results of our study on this larger than 5800-patient cohort suggest beneficial effects of high B12 vitamin level, negative effects of high sodium levels or high AST (aspartate aminotransferase) liver enzyme levels to cognition. PMID: 28918286
    3. Like in adults, FABP seems to be associated with markers of metabolic risk in obese adolescents PMID: 28183455
    4. Data suggest that, in context of abnormal hepatic lipid accumulation in nonalcoholic fatty liver disease, circulating GOT2 rises due to up-regulation of hepatic expression of GOT2 associated with greater gluconeogenesis and insulin resistance. PMID: 26791191
    5. A previously unknown mechanism by which GOT2 acetylation stimulates the malate-aspartate NADH shuttle activity and oxidative protection. PMID: 25755250
    6. High glucose infusion was associated with increased expression of higher plasma membrane-associated fatty acid-binding protein (FABPpm). PMID: 24937540
    7. The lipid binding process in L-FABP is associated with backbone dynamics. PMID: 22713574
    8. Frequency of polymorphism are similar in control groups and schizophrenics. PMID: 17728674

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  • 亚细胞定位：
    Mitochondrion matrix. Cell membrane.
  • 蛋白家族：
    Class-I pyridoxal-phosphate-dependent aminotransferase family
  • 数据库链接：

    HGNC: 4433

    OMIM: 138150

    KEGG: hsa:2806

    STRING: 9606.ENSP00000245206

    UniGene: Hs.599470

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