Human glucocorticoid receptor-β,GR-β ELISA Kit

产品详情

产品描述：

人糖皮质激素受体β(GR-β)酶联免疫试剂盒（CSB-E08889h）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的GR-β含量。GR-β即糖皮质激素受体β，是核受体超家族成员。它能拮抗GR-α转录活性，影响糖皮质激素效应。研究发现其在哮喘等炎症性疾病中异常表达，深入探究其作用机制，可为相关疾病的治疗提供新的靶点与思路。试剂盒检测范围为39 pg/mL-2500 pg/mL，适用于科研领域对GR-β表达水平的精准分析；可为体外研究GR-β在疾病模型中的表达调控、药物干预效果评估以及信号通路机制探索提供可靠工具；实验样本兼容性广泛，支持从基础细胞实验到动物组织研究的多样化需求，操作流程标准化，适用于实验室常规蛋白定量分析本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

lucocorticoid receptor-β,GR-β|lucocorticoid receptor-beta,GR-beta
缩写：

GR-β
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

39 pg/mL-2500 pg/mL
灵敏度：

9.75 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Epigenetics and Nuclear Signaling
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human GR-β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	103
	Range %	99-108
1:2	Average %	98
	Range %	94-102
1:4	Average %	91
	Range %	88-95
1:8	Average %	89
	Range %	85-96

回收率：

The recovery of human GR-β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	87	83-95

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.