Human flagellin ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CSB-E09671h人鞭毛蛋白（flagellin）酶联免疫试剂盒是一款基于夹心法原理设计的定量检测试剂盒，适用于检测人血清、血浆及组织匀浆样本中的鞭毛蛋白水平。鞭毛蛋白作为细菌鞭毛的核心结构成分，是触发宿主免疫应答的关键病原相关分子模式（PAMP），通过与Toll样受体5（TLR5）结合激活炎症信号通路，在细菌感染、肠道菌群调控及免疫相关疾病机制研究中具有重要价值。本试剂盒检测范围为0.78-50 ng/mL，灵敏度达0.78 ng/mL，可精准检测低丰度样本。其预包被高特异性抗体、优化的反应体系及标准品曲线，确保数据稳定性和重复性，支持多样本类型的高通量分析。该产品广泛应用于科研领域，如肠道炎症性疾病（如克罗恩病、溃疡性结肠炎）的机制研究、细菌感染模型中宿主-病原体相互作用评估，以及肠道微生物群与免疫调节相关性等基础研究，为探索鞭毛蛋白在先天免疫中的功能提供可靠工具。
缩写：

flagellin
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.78 ng/mL-50 ng/mL
灵敏度：

0.195 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Microbiology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human flagellin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	83-95
1:2	Average %	95
	Range %	91-99
1:4	Average %	95
	Range %	88-102
1:8	Average %	98
	Range %	91-104

回收率：

The recovery of human flagellin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	80-93
EDTA plasma (n=4)	95	92-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.