Human fetal hemoglobin,HBF ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

人胎儿血红蛋白(HBF)酶联免疫试剂盒（CSB-E09598h）为竞争法ELISA试剂盒，定量检测血清、血浆、RBC裂解物样本中的HBF含量。HBF（胎儿血红蛋白）具有重要背景。它在胎儿发育阶段是主要血红蛋白，出生后逐渐被成人血红蛋白替代。研究发现，提升 HBF 水平可改善某些血红蛋白病。其研究机制聚焦于调控 HBF 表达的基因与信号通路，以探索治疗相关疾病的新策略。试剂盒检测范围为125 ng/mL-2000 ng/mL，该产品适用于科研领域对胎儿血红蛋白表达调控机制的研究，如遗传性血红蛋白病的病理机制探索、造血干细胞分化过程中血红蛋白转换的分子研究，以及药物开发中对胎儿血红蛋白诱导疗法的效果评估，为血液学基础研究和疾病模型构建提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

HBF
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, lysate for RBC
检测范围：

125 ng/mL-2000 ng/mL
灵敏度：

62.5 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human HBF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	95
	Range %	91-99
1:40	Average %	103
	Range %	99-107
1:80	Average %	98
	Range %	93-103
1:160	Average %	92
	Range %	87-97

回收率：

The recovery of human HBF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	95-104
EDTA plasma (n=4)	89	84-94

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.