Human fetal Fibronectin(fFN)ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

人胎儿纤连蛋白(fFN)酶联免疫试剂盒（CSB-E17582h）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液、组织培养上清液样本中的fFN含量。fFN（胎儿纤维连接蛋白）是一种细胞外基质糖蛋白，主要分布在羊膜母胎面，绒毛膜和蜕膜之间。在正常情况下，fFN在宫颈阴道分泌物中的水平非常低，但在妊娠22周或之后，fFN水平升高与自发早产风险增加相关联。fFN检测可用于预测早产风险，其研究机制主要涉及fFN在妊娠和子宫之间的“黏合剂”作用，以及其在宫颈分泌物中的水平变化。试剂盒检测范围为25 ng/mL-1600 ng/mL，该产品适用于生殖医学领域的基础研究，例如妊娠相关生物标志物的体外分析、胎儿发育机制探索、病理妊娠模型的蛋白表达水平评估等科研场景，也可用于体外药物筛选或细胞模型中fFN调控机制的实验研究，为探索妊娠期分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

fFN
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, urine, cell culture supernates
检测范围：

25 ng/mL-1600 ng/mL
灵敏度：

6.25 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of Human fFN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	84-92
1:2	Average %	92
	Range %	84-96
1:4	Average %	94
	Range %	85-98
1:8	Average %	102
	Range %	98-105

回收率：

The recovery of Human fFN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	86-96
EDTA plasma (n=4)	89	82-95

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.