Human estrogen,E ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

本试剂盒（货号：CSB-E07286h）采用竞争法原理实现人雌激素（E）的精准定量检测，适用于血清和血浆样本的科研分析。雌激素作为人体重要的类固醇激素，参与调控生殖系统发育、骨骼代谢及脂质平衡等生理过程，其水平变化与内分泌功能、生殖健康密切相关。试剂盒检测范围为25-1000 pg/mL，通过优化的抗原抗体反应体系实现高灵敏检测，实验流程包含预包被抗体、样本孵育、竞争结合及显色读数等标准化步骤，操作时长约3小时。该产品可广泛应用于基础医学研究中激素动态监测、生殖生物学机制探索、环境内分泌干扰物评估，以及药物开发过程中雌激素相关代谢通路的体外分析，为实验室提供稳定可靠的检测工具。本产品仅限科学研究使用，需配合酶标仪（450nm波长）进行数据读取，建议通过标准曲线拟合实现样本浓度计算。
别名：

N/A
缩写：

E
种属：

Homo sapiens (Human)
样本类型：

serum, plasma
检测范围：

25 pg/mL-1000 pg/mL
灵敏度：

25 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human estrogen in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	88-101
1:2	Average %	94
	Range %	86-100
1:4	Average %	97
	Range %	94-101
1:8	Average %	91
	Range %	85-96

回收率：

The recovery of human estrogen spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-94
EDTA plasma (n=4)	96	92-100