Human deoxypyridinoline,DPD ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO人脱氧吡啶酚/脱氧吡啶啉（DPD）酶联免疫检测试剂盒（货号：CSB-E08399h），采用双抗体夹心法原理进行定量检测，适用于分析血清、尿液、细胞培养上清及组织匀浆样本中的DPD含量，检测灵敏度为1.56 ng/mL，线性范围覆盖1.56-100 ng/mL。DPD作为I型胶原蛋白交联降解的特异性产物，主要反映骨基质分解代谢水平，在骨吸收标志物研究中具有重要价值。本试剂盒通过预包被高亲和力抗体实现靶标捕获，结合酶标二抗显色系统完成定量分析，可满足骨代谢疾病机制研究、骨质疏松模型评估、骨折愈合监测或肿瘤骨转移实验等科研需求，其多类型样本兼容性为体外实验和动物研究提供灵活选择。该产品严格进行批次质控，配套提供标准品、检测抗体及全套实验缓冲液，适用于实验室常规骨代谢相关生物标志物的定量检测。
别名：

N/A
缩写：

DPD
种属：

Homo sapiens (Human)
样本类型：

serum, urine, cell culture supernates, tissue homogenates
检测范围：

1.56 ng/mL-100 ng/mL
灵敏度：

0.39 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human DPD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	80-92
1:2	Average %	94
	Range %	90-100
1:4	Average %	96
	Range %	91-106
1:8	Average %	92
	Range %	80-98

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.