Human cytokeratin fragment antigen 21-1,CYFRA21-1 ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

人细胞角蛋白21-1片段(CYFRA21-1)酶联免疫试剂盒（CSB-E09606h）为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的CYFRA21-1含量。CYFRA21-1是一种细胞角蛋白片段，广泛分布于正常组织中。当细胞癌变时，角蛋白被降解并释放到血液中，CYFRA21-1水平升高。它作为肺癌，尤其是鳞状细胞癌的标志物，可用于诊断和预后评估。试剂盒检测范围为0.312 ng/mL-20 ng/mL，本产品适用于基础医学研究中肿瘤标志物表达水平检测、疾病机制探索或药物开发过程中的生物标志物动态监测，为体外实验提供血清/血浆样本中CYFRA21 - 1浓度的可靠数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

CYFRA21-1
种属：

Homo sapiens (Human)
样本类型：

serum, plasma
检测范围：

0.312 ng/mL-20 ng/mL
灵敏度：

0.078 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CYFRA21-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	82-90
1:2	Average %	98
	Range %	91-105
1:4	Average %	99
	Range %	94-110
1:8	Average %	92
	Range %	86-99

回收率：

The recovery of human CYFRA21-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	80-98
EDTA plasma (n=4)	97	90-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.