Human cystathionine β-synthase(CBS) ELISA Kit

中文名称：

人胱硫醚β-合酶(CBS)酶联免疫试剂盒
货号：

CSB-E13314h
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

人胱硫醚β-合酶(CBS)酶联免疫试剂盒（CSB-E13314h）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CBS含量。人胱硫醚β-合酶（CBS）是转硫途径中的关键酶，参与同型半胱氨酸（Hcy）的代谢，其活性与心血管疾病风险相关。研究显示，CBS在Hcy转化为胱硫醚的转硫酸路径中起重要作用，其表达上调与多种疾病如唐氏综合症和癌症有关。抑制CBS可能对治疗这些疾病有益。试剂盒检测范围为125 pg/mL-8000 pg/mL，适用于研究CBS在细胞代谢调控、氧化应激响应及疾病模型中的表达变化，也可用于评估药物或基因干预对CBS活性的影响，为探究相关疾病的分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

AI047524 ELISA Kit; AI303044 ELISA Kit; Beta thionase ELISA Kit; Beta-thionase ELISA Kit; Cbs ELISA Kit; Cbs cystathionine beta-synthase ELISA Kit; CBS_HUMAN ELISA Kit; Cystathionine beta synthase ELISA Kit; Cystathionine beta-synthase ELISA Kit; EC 4.2.1.22 ELISA Kit; HIP 4 ELISA Kit; HIP4 ELISA Kit; Methylcysteine synthase ELISA Kit; MGC18856 ELISA Kit; MGC18895 ELISA Kit; MGC37300 ELISA Kit; OTTHUMP00000109416 ELISA Kit; OTTHUMP00000109418 ELISA Kit; Serine sulfhydrase ELISA Kit
缩写：

CBS
Uniprot No.：

P35520
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

125 pg/mL-8000 pg/mL
灵敏度：

31.25 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CBS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-98
1:2	Average %	106
	Range %	102-110
1:4	Average %	96
	Range %	90-101
1:8	Average %	88
	Range %	86-96

回收率：

The recovery of human CBS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	98-106
EDTA plasma (n=4)	94	91-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
8000	2.144	2.033	2.089	1.978
4000	1.388	1.411	1.400	1.289
2000	0.932	0.989	0.961	0.850
1000	0.655	0.636	0.646	0.535
500	0.445	0.424	0.435	0.324
250	0.266	0.268	0.267	0.156
125	0.212	0.221	0.217	0.106
0	0.110	0.112	0.111
		.

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

引用文献

Maternal vitamin D deficiency impact on LCPUFA and pregnancy outcome associated with alterations in the one carbon metabolism AA Nandi,Nutrition Research,2020
Potential biomarkers predicting risk of pulmonary hypertension in congenital heart disease: the role of homocysteine and hydrogen sulfide Sun L et al,Chin Med J (Engl),2014

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功能：

Hydro-lyase catalyzing the first step of the transsulfuration pathway, where the hydroxyl group of L-serine is displaced by L-homocysteine in a beta-replacement reaction to form L-cystathionine, the precursor of L-cysteine. This catabolic route allows the elimination of L-methionine and the toxic metabolite L-homocysteine. Also involved in the production of hydrogen sulfide, a gasotransmitter with signaling and cytoprotective effects on neurons.
相关疾病：

Cystathionine beta-synthase deficiency (CBSD)
亚细胞定位：

Cytoplasm. Nucleus.
蛋白家族：

Cysteine synthase/cystathionine beta-synthase family
组织特异性：

In the adult strongly expressed in liver and pancreas, some expression in heart and brain, weak expression in lung and kidney. In the fetus, expressed in brain, liver and kidney.
数据库链接：

HGNC: 1550

OMIM: 236200

KEGG: hsa:102724560

UniGene: Hs.533013

Human cystathionine β-synthase(CBS) ELISA Kit

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