Human cross linked N-telopeptide of type Ⅰ collagen,NTX ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO人Ⅰ型胶原N末端肽（NTX）酶联免疫检测试剂盒（货号：CSB-E09233h），适用于血清、血浆及尿液样本中NTX的定量分析。NTX是Ⅰ型胶原蛋白降解过程中释放的特异性片段，可作为骨吸收过程的生物标志物，在骨质疏松症、骨转移癌等骨骼代谢异常相关研究中具有重要价值。本试剂盒基于双抗体夹心法原理，通过预包被高特异性抗体实现目标蛋白的捕获与检测，检测灵敏度为7.8 nM BCE，线性范围覆盖7.8 nM BCE至500 nM BCE，可精准定量不同浓度梯度的样本。该产品适用于骨代谢机制研究、抗骨质疏松药物开发评估、骨骼疾病动物模型分析等科研领域，其多样本兼容性特点支持研究者灵活选择体液样本进行横向比对实验。实验操作全程在常规实验室条件下进行，配套提供标准品与优化试剂，确保检测结果的重现性和准确性，为骨代谢相关基础研究提供可靠工具。
别名：

cross linked N-telopeptide of type 1 collagen,NTX,_x000D_ cross linked N-telopeptide of type I collagen,NTX
缩写：

NTX
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, urine
检测范围：

7.8 nM BCE-500 nM BCE
灵敏度：

1.95 nM BCE
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human NTX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-93
1:2	Average %	98
	Range %	94-102
1:4	Average %	102
	Range %	98-106
1:8	Average %	95
	Range %	92-99

回收率：

The recovery of human NTX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	85-106
EDTA plasma (n=4)	92	88-101

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.