Human cleaved microtubule-associated protein tau (C-MAPT/C-TAU) ELISA kit

Human cleaved microtubule-associated protein tau (C-MAPT/C-TAU) ELISA kit

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中文名称：

人裂解微管相关蛋白tau(C-MAPT/C-TAU)酶联免疫试剂盒
货号：

CSB-ECL013481H
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO定量检测人血清、血浆、脑脊液、组织匀浆及细胞裂解液样本中裂解微管相关蛋白tau（C-MAPT/C-TAU）的酶联免疫试剂盒（货号CSB-ECL013481H），采用双抗体夹心法原理，通过特异性抗体对靶蛋白进行高灵敏度检测，线性范围为62.5-4000 pg/mL，适用于科研领域对tau蛋白的浓度分析。Tau蛋白作为神经元微管稳定的关键调节因子，其异常聚集与阿尔茨海默病、额颞叶痴呆等神经退行性疾病密切相关，尤其在脑脊液和脑组织中的病理积累是疾病进展的重要生物标志物。该试剂盒支持多种生物样本类型的检测，可用于评估疾病模型中的tau蛋白动态变化、探究神经退行机制及药物干预效果研究，为神经病理学、分子生物学实验提供可靠工具，其宽泛的检测范围可满足低丰度样本分析及高浓度样本梯度稀释需求，实验流程标准化且重复性良好，适用于实验室基础研究与转化医学探索。
别名：

cleaved form of DDPAC, FLJ31424, FTDP-17, MAPTL, MGC138549, MSTD, MTBT1, MTBT2, PPND, TAU, G protein beta1/gamma2 subunit-interacting factor 1|microtubule-associated protein tau, isoform 4
缩写：

C-MAPT/C-TAU
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, cerebrospinal fluid (CSF), tissue homogenates, cell lysates
检测范围：

62.5 pg/mL-4000 pg/mL
灵敏度：

15.6 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human C-MAPT/C-TAU in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	102
	Range %	96-106
1:10	Average %	100
	Range %	90-104
1:20	Average %	95
	Range %	85-99
1:40	Average %	94
	Range %	88-99

回收率：

The recovery of human C-MAPT/C-TAU spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	82-95
EDTA plasma (n=4)	100	91-104

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	3.001	2.889	2.945	2.832
2000	2.114	1.976	2.045	1.932
1000	1.231	1.200	1.216	1.103
500	0.596	0.565	0.581	0.468
250	0.353	0.341	0.347	0.234
125	0.239	0.232	0.236	0.123
62.5	0.171	0.168	0.170	0.057
0	0.111	0.115	0.113

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

A prospective pilot study on serum cleaved tau protein as a neurological marker in severe traumatic brain injury Pandey S.et al,Br J Neurosurg,2017

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.